Isothermal Amplification of Genetically Modified DNA Sequences Directly from Plant Tissues Lowers the Barriers to High-Throughput and Field-Based Genotyping

被引：13
作者：
Lee, David [1 ]
La Mura, Maurizio [1 ]
Allnutt, Theo [2 ]
Powell, Wayne
Greenland, Andy [1 ]
机构：
[1] NIAB, John Bingham Lab, Cambridge CB3 0LE, England
[2] Cent Sci Lab, York YO41 1LZ, N Yorkshire, England
来源：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2009年 / 57卷 / 20期
关键词：
Loop-mediated isothermal amplification (LAMP); GM detection; RF3 oilseed rape; leaf tissue; MON810 maize seed; LEAF;
D O I：
10.1021/jf902455j
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
DNA extractions are a major cost for high-throughput genotyping. The loop-mediated isothermal amplification (LAMP) assay has been used for the detection of two genetically modified (GM) related sequences. The amplification of target DNA sequences from leaf and maize seed tissues prepared with minimum preparative treatment (disruption in water) demonstrates the ability of LAMP to work in conditions normally inhibitive to PCRs. The wide dynamic range of detection in these samples suggests that LAMP is highly sensitive even when the target is presented in such a crude form. LAMP offers a means of reducing genotyping costs as well as simplifying testing procedures.
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收藏
页码：9400 / 9402
页数：3
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