The Visualizing Agents for Selected Quinolones and Fluoroquinolones

Analysis of compounds having biological activity is very important nowadays. This work shows the possibility of using the visualizing agents such as janus blue, methylene violet, gentian violet, methyl green, cresol red, rodamine B, malachite green, methylene blue, eosin yellowish, and metanil yellow to visualize the selected quinolones and fluoroquinolones after their separation using the TLC method. The visualizing effects were estimated after dipping the chromatographic plates in solutions of the mentioned above visualizing agents and drying at room temperature, at 120 degrees C, or was estimated directly after dipping in solutions of the visualizing agents. The detection limit of quinolones and fluoroquinolones investigated in the conditions of visualization that gave the best visualizing effects was determined. The detection limits for cinoxacin, pipemidic acid, ofloxacin, and pefloxacin were: 0.1 mu g, 0.1 mu g, 0.5 mu g, and 0.75 mu g, respectively.