2,6,8-Trisubstituted 3-Hydroxychromone Derivatives as Fluorophores for Live-Cell Imaging

被引：28

作者：

Dyrager, Christine ^{[1
]}

Friberg, Annika ^{[1
]}

Dahlen, Kristian ^{[1
]}

Friden-Saxin, Maria ^{[1
]}

Borjesson, Karl ^{[2
]}

Wilhelmsson, L. Marcus ^{[2
]}

Smedh, Maria ^{[3
]}

Grotli, Morten ^{[1
]}

Luthman, Kristina ^{[1
]}

机构：

[1] Univ Gothenburg, Dept Chem Med Chem, S-41296 Gothenburg, Sweden

[2] Chalmers, Dept Chem & Biol Engn Phys Chem, S-41296 Gothenburg, Sweden

[3] Univ Gothenburg, Sahlgrenska Acad, Ctr Cellular Imaging, S-40530 Gothenburg, Sweden

来源：

CHEMISTRY-A EUROPEAN JOURNAL | 2009年 / 15卷 / 37期

基金：

瑞典研究理事会;

关键词：

cellular imaging; excited-state intramolecular proton transfer (ESIPT); fluorescence; fluorophores; hydroxychromones; STATE PROTON-TRANSFER; INTRAMOLECULAR EXCITED-STATE; PHOSPHOLIPID-MEMBRANES; FLUORESCENCE PROBES; SURFACE-CHARGE; DUAL EMISSION; 3-HYDROXYFLAVONE; DYES; PERTURBATION; HYDRATION;

D O I：

10.1002/chem.200900279

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We present the synthesis and photophysical characterisation of a series of structurally diverse, fluorescent 2,6,8-trisubstituted 3-hydroxychromone derivatives with high fluorescence quantum yields and molar extinction coefficients. Two of these derivatives (9 and 10a) have been studied as fluorophores for cellular imaging in HeLa cells and show excellent permeability and promising fluorescence properties in a cellular environment. In addition, we have demonstrated by photophysical characterisation of 3-isobutyroxychromone derivatives that esterification of the 3-hydroxyl group results in acceptable and useful fluorescence properties.

引用

页码：9417 / 9423

页数：7

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