Rapid detection of blaKPC directly from surveillance rectal swabs by EasyQ KPC

被引：3
作者：
Ramos, Ana Carolina [1 ]
Schandert, Lygia [1 ]
Carvalhaes, Cecilia G. [1 ,2 ]
Martins, Rafael Affini [1 ]
Cappellano, Paola [3 ]
Machado, Flavia Ribeiro [4 ]
Gales, Ana Cristina [1 ]
机构：
[1] Univ Fed Sao Paulo, Escola Paulista Med, Dept Internal Med, Div Infect Dis,Lab Alerta, Sao Paulo, Brazil
[2] Univ Fed Sao Paulo, Escola Paulista Med, Div Lab Med, Lab Cent,Hosp Sao Paulo,Internal Med, Sao Paulo, Brazil
[3] Univ Fed Sao Paulo, Disciplina Infectol, Sao Paulo, Brazil
[4] Univ Fed Sao Paulo, Disciplina Anestesiol Dor & Med Intens, Sao Paulo, Brazil
来源：
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2018年 / 90卷 / 04期
关键词：
EasyQ KPC; Rectal swabs; PCR; blaKPC; Enterobacteriaceae; CARBAPENEMASE-PRODUCING ENTEROBACTERIACEAE; KLEBSIELLA-PNEUMONIAE;
D O I：
10.1016/j.diagmicrobio.2017.12.009
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
The performance of EasyQ KPC assay was evaluated for the first time for bla(KPC) detection directly from surveillance rectal swabs without broth enrichment. Using conventional polymerase chain reaction as gold standard method, EasyQ KPC and culture-based molecular tests demonstrated a sensitivity/specificity of 100%/87.3% and 83.3%/98.2%, respectively. (C) 2017 Elsevier Inc. All rights reserved.
引用
收藏
页码：251 / 252
页数：2
相关论文
共 9 条
  • [1] Klebsiella pneumoniae ST258 Producing KPC-3 Identified in Italy Carries Novel Plasmids and OmpK36/OmpK35 Porin Variants
    Garcia-Fernandez, Aurora
    Villa, Laura
    Carta, Claudio
    Venditti, Carolina
    Giordano, Alessandra
    Venditti, Mario
    Mancini, Carlo
    Carattoli, Alessandra
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2012, 56 (04) : 2143 - 2145
  • [2] Global Dissemination of Carbapenemase-Producing Klebsiella pneumoniae: Epidemiology, Genetic Context, Treatment Options, and Detection Methods
    Lee, Chang-Ro
    Lee, Jung Hun
    Park, Kwang Seung
    Kim, Young Bae
    Jeong, Byeong Chul
    Lee, Sang Hee
    [J]. FRONTIERS IN MICROBIOLOGY, 2016, 7
  • [3] Direct Ertapenem Disk Screening Method for Identification of KPC-Producing Klebsiella pneumoniae and Escherichia coli in Surveillance Swab Specimens
    Lolans, Karen
    Calvert, Karen
    Won, Sarah
    Clark, James
    Hayden, Mary K.
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2010, 48 (03) : 836 - 841
  • [4] Evaluation of the NucliSENS EasyQ KPC Assay for Detection of Klebsiella pneumoniae Carbapenemase-Producing Enterobacteriaceae
    McEwan, A. S.
    Derome, A.
    Meunier, D.
    Burns, P. J.
    Woodford, N.
    Dodgson, A. R.
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2013, 51 (06) : 1948 - 1950
  • [5] First Report of KPC-2-Producing Klebsiella pneumoniae Strains in Brazil
    Monteiro, Jussimara
    Santos, Anderson Fernandes
    Asensi, Marise Dutra
    Peirano, Gisele
    Gales, Ana Cristina
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2009, 53 (01) : 333 - 334
  • [6] Rapid and sensitive detection of blaKPC gene in clinical isolates of Klebsiella pneumoniae by a molecular real-time assay
    Mosca, Adriana
    Miragliotta, Luisa
    Del Prete, Raffaele
    Tzakis, Gerasimos
    Dalfino, Lidia
    Bruno, Francesco
    Pagani, Laura
    Migliavacca, Roberta
    Piazza, Aurora
    Miragliotta, Giuseppe
    [J]. SPRINGERPLUS, 2013, 2 : 1 - 5
  • [7] Clinical epidemiology of the global expansion of Klebsiella pneumoniae carbapenemases
    Munoz-Price, L. Silvia
    Poirel, Laurent
    Bonomo, Robert A.
    Schwaber, Mitchell J.
    Daikos, George L.
    Cormican, Martin
    Cornaglia, Giuseppe
    Garau, Javier
    Gniadkowski, Marek
    Hayden, Mary K.
    Kumarasamy, Karthikeyan
    Livermore, David M.
    Maya, Juan J.
    Nordmann, Patrice
    Patel, Jean B.
    Paterson, David L.
    Pitout, Johann
    Villegas, Maria Virginia
    Wang, Hui
    Woodford, Neil
    Quinn, John P.
    [J]. LANCET INFECTIOUS DISEASES, 2013, 13 (09) : 785 - 796
  • [8] Counting the cost of an outbreak of carbapenemase-producing Enterobacteriaceae: an economic evaluation from a hospital perspective
    Otter, J. A.
    Burgess, P.
    Davies, F.
    Mookerjee, S.
    Singleton, J.
    Gilchrist, M.
    Parsons, D.
    Brannigan, E. T.
    Robotham, J.
    Holmes, A. H.
    [J]. CLINICAL MICROBIOLOGY AND INFECTION, 2017, 23 (03) : 188 - 196
  • [9] Evaluation of the New NucliSENS EasyQ KPC Test for Rapid Detection of Klebsiella pneumoniae Carbapenemase Genes (blaKPC)
    Spanu, Teresa
    Fiori, Barbara
    D'Inzeo, Tiziana
    Canu, Giulia
    Campoli, Serena
    Giani, Tommaso
    Palucci, Ivana
    Tumbarello, Mario
    Sanguinetti, Maurizio
    Rossolini, Gian Maria
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2012, 50 (08) : 2783 - 2785
1