Applications of Luminous Bacteria Enzymes in Toxicology

被引：45

作者：

Kratasyuk, Valentina A. ^{[1
,2
]}

Esimbekova, Elena N. ^{[1
,2
]}

机构：

[1] Siberian Fed Univ, Krasnoyarsk 660041, Russia

[2] Russian Acad Sci, Inst Biophys, Siberian Branch, Photobiol Lab, Krasnoyarsk 660036, Russia

来源：

COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING | 2015年 / 18卷 / 10期

基金：

俄罗斯科学基金会;

关键词：

Bioluminescence; bioluminescent toxicity enzymatic assay; immobilization of enzymes; luciferase; total toxicity; HUMIC SUBSTANCES; DETOXIFICATION PROCESSES; BIOLUMINESCENT ASSAY; LUCIFERASE; SYSTEM; STABILIZERS; INHIBITION; TOXICITY; QUINONES; STARCH;

D O I：

10.2174/1386207318666150917100257

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This review describes the principle and applications of bioluminescent enzymatic toxicity bioassays. This type of assays uses bacterial coupled enzyme systems: NADH: FMN-oxidoreductase and luciferase to replace living organisms in developing cost-competitive biosensors for environmental, medical and industrial applications. These biosensors instantly signal chemical and biological hazards and allow for detecting a great amount of toxic compounds with advantages associated with fast results, high sensitivity, simplicity, low cost and safety of the procedure.

引用

页码：952 / 959

页数：8