Reaction-based probes for Co(II) and Cu(I) with dual output modes: fluorescence live cell imaging

被引：58

作者：

Maity, Debabrata ^{[1
]}

Raj, Anand ^{[1
]}

Karthigeyan, D. ^{[2
]}

Kundu, Tapas K. ^{[2
]}

Govindaraju, T. ^{[1
]}

机构：

[1] Jawaharlal Nehru Ctr Adv Sci Res, New Chem Unit, Bioorgan Chem Lab, Bangalore 560064, Karnataka, India

[2] Jawaharlal Nehru Ctr Adv Sci Res, Mol Biol & Genet Unit, Bangalore 560064, Karnataka, India

来源：

RSC ADVANCES | 2013年 / 3卷 / 37期

关键词：

LIVING CELLS; RATIOMETRIC DETECTION; METAL-IONS; COBALT; COPPER; SENSORS; CO2+; CU2+; AMPLIFICATION; CHEMOSENSOR;

D O I：

10.1039/c3ra41588k

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Two new reaction-based molecular probes ResCo and ResCu are reported for the selective colourimetric and fluorometric detection of Co2+ and Cu+, respectively. The receptor in these probes differs by a single metal coordinating site combined with resorufin as a common reporter dye, which exhibit visible absorption and emission in the longer wavelength regions. Co2+- and Cu+-mediated oxidative benzylic ether (C-O) bond cleavage in ResCo and ResCu, respectively, releases resorufin dye which offer 'switch-on' fluorometric and ratiometric visible absorption (colourimetric) in a reducing aqueous environment. Reaction-based selective detection of Co2+ and Cu+ is demonstrated in the presence of all other competing metal ions. Submicromolar concentration of Co2+ and Cu+ can be easily detected through dual signaling (fluorometric and colourimetric) pathways. The probes ResCo and ResCu are employed for 'switch-on' fluorescence live cell imaging of Co2+ and Cu+, respectively, in HEK293T cells.

引用

页码：16788 / 16794

页数：7

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