Kinetic studies on the hydrolysis of soluble and cassava starches by maltogenase

In view of utilizing the commercial enzyme Maltogenase for the production of maltose syrup in a continuous membrane reactor, some kinetic properties of this enzyme were investigated. Preliminary test were conducted using Zulkowsky soluble starch as substrate. At pH 5.0 and 6.0 degrees C, the Michaelis and Menten kinetic constants V-max and K-M were respectively 2.47mmol of maltose equivalents.min(-1).dm(-3) and 9.64g of Zulkowsky soluble starch.dm(-3). Initial hydrolysis velocity was reduced at high starch concentrations and in the presence of maltose. When liquefield cassava starch (approximate to 60 to 340g.dm(-3)) was used as substrate, hydrolysis kinetics was modelled by analyzing whole progress curves. A simple empirical modell was established and allowed satisfactory descriptions of the experimental data for the formation of maltose over time at large enzyme/substrate rations.