Methotrexate loaded gellan gum microparticles for drug delivery

被引：54
作者：
Dhanka, Mukesh [1 ]
Shetty, Chaitra [1 ]
Srivastava, Rohit [1 ]
机构：
[1] Indian Inst Technol, Dept Biosci & Bioengn, Bombay 400076, Maharashtra, India
来源：
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2018年 / 110卷
关键词：
Methotrexate; Gellan gum; Microparticles; Cell viability; Hemolysis; POLY CAPROLACTONE MICROPARTICLES; IN-VIVO EVALUATION; ALGINATE MICROPARTICLES; ENHANCED DISPERSION; VITRO RELEASE; MICROSPHERES; NANOPARTICLES; FORMULATION; ARTHRITIS; SYSTEM;
D O I：
10.1016/j.ijbiomac.2017.12.026
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Recently, polysaccharides based microparticles have been found to offer an attractive potential as a carrier in drug delivery field. In this study, bare gellan gum microparticles (GG MPs) and methotrexate (MTX) loaded gellan gum microparticles (MTX-GG MPs) prepared by using simple water-in-oil (W/O) emulsion solvent diffusion method. The developed microparticles (MPs) were found discretely distributed in a spherical shape. MTX has been encapsulated in microparticles with 84.8 +/- 1.68% encapsulation efficiency (%EE) and 6.45 +/- 0.07% loading capacity (%LC). The Fourier Transform Infrared Spectroscopy (FTIR) characterization of the MPs clearly indicated the physical encapsulation of MTX into polymeric matrix of MPs. Thermogravimetric analysis (TGA) characterization showed slightly higher thermal stability of MTX-GG MPs in comparison to the GG MPs. In vitro release study of MTX-GG MPs showed 84% drug release within 24 h. The hemolysis study of GG MPs and MTX-GG MPs on human red blood cells (RBCs) showed <1.0% hemolysis. The cell viability studies on L929 showed GG MPs, and MTX-GG MPs are biocompatible. (C) 2017 Elsevier B.V. All rights reserved.
引用
收藏
页码：346 / 356
页数：11
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