Scale-up micropropagation of sweet basil (Ocimum basilicum L.) in an airlift bioreactor and accumulation of rosmarinic acid

被引：27

作者：

Kintzios, S ^{[1
]}

Kollias, H ^{[1
]}

Straitouris, E ^{[1
]}

Makri, O ^{[1
]}

机构：

[1] Agr Univ Athens, Fac Agr Biotechnol, Lab Plant Physiol, Athens 11855, Greece

来源：

BIOTECHNOLOGY LETTERS | 2004年 / 26卷 / 06期

关键词：

airlift bioreactor; micropropagation; Ocimum basilicum; rosmarinic acid;

D O I：

10.1023/B:BILE.0000019561.89044.30

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Nodal explants with lateral buds and leaf-derived suspension cultures of sweet basil, Ocimum basilicum L., were cultured in 5 l airlift bioreactors for three weeks, thereby increasing the fresh wt of suspension cultures 2.5-fold. Rosmarinic acid accumulated at 29 mug g(-1) dry wt in the suspension culture but, for micropropagated plants, it reached 178 mug g(-1) dry wt.

引用

页码：521 / 523

页数：3

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