Scale-up micropropagation of sweet basil (Ocimum basilicum L.) in an airlift bioreactor and accumulation of rosmarinic acid

被引：27
|
作者：
Kintzios, S [1 ]
Kollias, H [1 ]
Straitouris, E [1 ]
Makri, O [1 ]
机构：
[1] Agr Univ Athens, Fac Agr Biotechnol, Lab Plant Physiol, Athens 11855, Greece
来源：
BIOTECHNOLOGY LETTERS | 2004年 / 26卷 / 06期
关键词：
airlift bioreactor; micropropagation; Ocimum basilicum; rosmarinic acid;
D O I：
10.1023/B:BILE.0000019561.89044.30
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Nodal explants with lateral buds and leaf-derived suspension cultures of sweet basil, Ocimum basilicum L., were cultured in 5 l airlift bioreactors for three weeks, thereby increasing the fresh wt of suspension cultures 2.5-fold. Rosmarinic acid accumulated at 29 mug g(-1) dry wt in the suspension culture but, for micropropagated plants, it reached 178 mug g(-1) dry wt.
引用
收藏
页码：521 / 523
页数：3
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