Immunolocalization of FGF-2,-7,-8,-10 and FGFR-1-4 during regeneration of the rat submandibular gland

被引:8
作者
Shimizu, Osamu [1 ,2 ]
Yasumitsu, Tomohiro [1 ,3 ]
Shiratsuchi, Hiroshi [1 ,2 ]
Oka, Shunichi [4 ]
Watanabe, Tatsuhisa [1 ,2 ]
Saito, Tadahito [1 ,2 ]
Yonehara, Yoshiyuki [1 ,2 ]
机构
[1] Nihon Univ, Sch Dent, Dept Oral & Maxillofacial Surg, Chiyoda Ku, Tokyo 1018310, Japan
[2] Nihon Univ, Sch Dent, Div Syst Biol & Oncol, Tokyo 1018310, Japan
[3] Nihon Univ, Grad Sch Dent, Div Oral Struct & Funct Biol, Tokyo 1018310, Japan
[4] Nihon Univ, Sch Dent, Dept Anesthesiol, Tokyo 1018310, Japan
关键词
Fibroblast growth factor (FGF); Fibroblast growth factor receptor (FGFR); Rat submandibular gland; Regeneration; Duct-like structure (DLS); Immunohistochemistry; FIBROBLAST-GROWTH-FACTOR; SALIVARY-GLANDS; IMMUNOHISTOCHEMICAL LOCALIZATION; BRANCHING MORPHOGENESIS; CELL-PROLIFERATION; FACTOR RECEPTOR-3; ESSENTIAL ROLES; EXPRESSION; FGF; MICE;
D O I
10.1007/s10735-015-9631-6
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Fibroblast growth factors (FGFs) and their receptors (FGFRs) play important roles in the development of the submandibular gland. Although regeneration of submandibular glands follows a similar process to their development, it is unknown how FGFs and FGFRs are distributed during regeneration of submandibular gland. The aim of this study was to determine the localization of FGFs and FGFRs during such regenerative processes. After 7 days' obstruction, the submandibular glands were collected at days 0, 1, 3, 7, 11 and 14 after duct release to study regeneration. The regenerative processes of the submandibular gland were investigated by immunohistochemistry for FGF-2, 7, 8, 10 and FGFR-1-4. Immunohistochemical staining revealed that FGF-2 was moderately expressed in the epithelial cells of duct-like structures (DLS) and newly formed acinar cells (NFAC) at days 0-7, and strongly in intercalated duct (ICD) at control gland and Day 7-14. FGF-7 was localized moderately in NFAC and DLS. FGF-8 was localized moderately in the epithelial cells of DLS during regeneration. Strong positive immunoreactions for FGF-10 were found in NFAC and the epithelial cells of DLS during regeneration, as well as the ICD and lateral surfaces of the maturing acinar cells (MAC). FGFR-1 was expressed moderately in the ICD, and weakly in the NFAC and MAC. Positive immunoreactions for FGFR-2 were not observed during regeneration. Additionally, FGFR-4 was detected strongly in the ICD and slightly in NFAC. These findings suggest that FGF-2, -7, -8 and -10 play important roles in NFAC, MAC, and DLS through FGFR-1 and -4 during regeneration of submandibular gland.
引用
收藏
页码:421 / 429
页数:9
相关论文
共 41 条
[1]   BASIC FIBROBLAST GROWTH-FACTOR IN RAT SALIVARY-GLANDS [J].
AMANO, O ;
YOSHITAKE, Y ;
NISHIKAWA, K ;
ISEKI, S .
CELL AND TISSUE RESEARCH, 1993, 273 (03) :467-474
[2]   Aquaporins in the adult mouse submandibular and sublingual salivary glands [J].
Aure, Marit H. ;
Ruus, Ann-Kristin ;
Galtung, Hilde K. .
JOURNAL OF MOLECULAR HISTOLOGY, 2014, 45 (01) :69-80
[3]   REGENERATION OF SALIVARY GLANDS IN RABBIT [J].
BHASKAR, SN ;
LILLY, GE ;
BHUSSRY, B .
JOURNAL OF DENTAL RESEARCH, 1966, 45 (01) :37-&
[4]   Myoepithelial cells actively proliferate during atrophy of rat parotid gland [J].
Burgess, KL ;
Dardick, L ;
Cummins, MM ;
BurfordMason, AP ;
Bassett, R ;
Brown, DH .
ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTICS, 1996, 82 (06) :674-680
[5]   Soluble dominant-negative receptor uncovers essential roles for fibroblast growth factors in multi-organ induction and patterning [J].
Celli, G ;
LaRochelle, WJ ;
Mackem, S ;
Sharp, R ;
Merlino, G .
EMBO JOURNAL, 1998, 17 (06) :1642-1655
[6]   Skeletal overgrowth and deafness in mice lacking fibroblast growth factor receptor 3 [J].
Colvin, JS ;
Bohne, BA ;
Harding, GW ;
McEwen, DG ;
Ornitz, DM .
NATURE GENETICS, 1996, 12 (04) :390-397
[7]  
De Moerlooze L, 2000, DEVELOPMENT, V127, P483
[8]   MURINE FGFR-1 IS REQUIRED FOR EARLY POSTIMPLANTATION GROWTH AND AXIAL ORGANIZATION [J].
DENG, CX ;
WYNSHAWBORIS, A ;
SHEN, MM ;
DAUGHERTY, C ;
ORNITZ, DM ;
LEDER, P .
GENES & DEVELOPMENT, 1994, 8 (24) :3045-3057
[9]   Fibroblast growth factor receptor 3 is a negative regulator of bone growth [J].
Deng, CX ;
WynshawBoris, A ;
Zhou, F ;
Kuo, A ;
Leder, P .
CELL, 1996, 84 (06) :911-921
[10]   Keratinocyte growth factor is required for hair development but not for wound healing [J].
Guo, LF ;
Degenstein, L ;
Fuchs, E .
GENES & DEVELOPMENT, 1996, 10 (02) :165-175