Portable Guided-Mode Resonance Biosensor Platform for Point-of-care Testing

被引:5
作者
Sung, Gun Yong [1 ]
Kim, Wan-Joong [1 ]
Ko, Hyunsung [1 ]
Kim, Bong Kyu [1 ]
Kim, Kyung-Hyun [1 ]
Huh, Chul [1 ]
Hong, Jongcheol [1 ]
机构
[1] Elect & Telecommun Res Inst, Taejon 305700, South Korea
来源
BIOSENSING AND NANOMEDICINE V | 2012年 / 8460卷
关键词
Guided Mode Resonance Filter; Biosensor; Point-of-care testing; label-free; Cardiac; Immunoassay; Tunable VCSEL; Nano imprinting; LABEL-FREE;
D O I
10.1117/12.929457
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
It represents a viable solution for the realization of a portable biosensor platform that could screen/diagnose acute myocardial infarction by measuring cardiac marker concentrations such as cardiac troponin I (cTnI), creatine kinase MB (CK-MB), and myoglobin (MYO) for application to u-health monitoring system. The portable biosensor platform introduced in this presentation has a more compact structure and a much higher measuring resolution than a conventional spectrometer system. Portable guided-mode resonance (GMR) biosensor platform was composed of a biosensor chip stage, an optical pick-up module, and a data display panel. Disposable plastic GMR biosensor chips with nano-grating patterns were fabricated by injection-molding. Whole blood filtration and label-free immunoassay were performed on these single chips, automatically. Optical pick-up module was fabricated by using the miniaturized bulk optics and the interconnecting optical fibers and a tunable VCSEL (vertical cavity surface emitting laser). The reflectance spectrum from the GMR biosensor was measured by the optical pick-up module. Cardiac markers in human serum with concentrations less than 0.1ng/mL were analyzed using a GMR biosensor. Analysis time was 30min, which is short enough to meet clinical requirements. Our results show that the GMR biosensor will be very useful in developing low-cost portable biosensors that can screen for cardiac diseases.
引用
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页数:14
相关论文
共 18 条
[1]   Label-free, single-molecule detection with optical microcavities [J].
Armani, Andrea M. ;
Kulkarni, Rajan P. ;
Fraser, Scott E. ;
Flagan, Richard C. ;
Vahala, Kerry J. .
SCIENCE, 2007, 317 (5839) :783-787
[2]   Troponin: the biomarker of choice for the detection of cardiac injury [J].
Babuin, L ;
Jaffe, AS .
CANADIAN MEDICAL ASSOCIATION JOURNAL, 2005, 173 (10) :1191-1202
[3]  
BHAYANA V, 1993, LANCET, V342, P1554, DOI 10.1016/S0140-6736(05)80123-0
[4]   Surface plasmon resonance sensors: review [J].
Homola, J ;
Yee, SS ;
Gauglitz, G .
SENSORS AND ACTUATORS B-CHEMICAL, 1999, 54 (1-2) :3-15
[5]   Prediction of the limit of detection of an optical resonant reflection biosensor [J].
Hong, Jongcheol ;
Kim, Kyung-Hyun ;
Shin, Jae-Heon ;
Huh, Chul ;
Sung, Gun Yong .
OPTICS EXPRESS, 2007, 15 (14) :8972-8978
[6]   Frequency Swept Laser at 1300 nm Using a Wavelength Scanning Filter Based on a Rotating Slit Disk [J].
Jeon, Mansik ;
Jung, Unsang ;
Song, Jae-Won ;
Kim, Jeehyun ;
Oh, Junghwan ;
Eom, Jinseob ;
Kim, Chang-Seok ;
Park, Young-Ho .
JOURNAL OF THE OPTICAL SOCIETY OF KOREA, 2009, 13 (03) :330-334
[7]   Fabrication of a Nanosize Pattern Embedded Plastic Chip via an Injection Molding Method for Application to an Optical Biosensor [J].
Kim, K. H. ;
Kim, W. ;
Hong, J. C. ;
Ko, H. S. ;
Kim, B. K. ;
Huh, C. ;
Sung, G. Y. .
INTERNATIONAL POLYMER PROCESSING, 2010, 25 (05) :341-345
[8]   Response to Cardiac Markers in Human Serum Analyzed by Guided-Mode Resonance Biosensor [J].
Kim, Wan-Joong ;
Kim, Bong Kyu ;
Kim, Ansoon ;
Huh, Chul ;
Ah, Chil Seong ;
Kim, Kyung-Hyun ;
Hong, Jongcheol ;
Park, Seon Hee ;
Song, Sanghoon ;
Song, Junghan ;
Sung, Gun Yong .
ANALYTICAL CHEMISTRY, 2010, 82 (23) :9686-9693
[9]   A Compact Tunable VCSEL and a Built-in Wavelength Meter for a Portable Optical Resonant Reflection Biosensor Reader [J].
Ko, Hyunsung ;
Kim, Bong Kyu ;
Kim, Kyung Hyun ;
Huh, Chul ;
Kim, Wan-Joong ;
Hong, Jongcheol ;
Park, Seon Hee ;
Yang, Seong-Seok ;
Jang, Ho-Jin ;
Sung, Gun Yong .
JOURNAL OF THE OPTICAL SOCIETY OF KOREA, 2010, 14 (04) :395-402
[10]   Widely tunable grating cavity lasers [J].
Kwon, Oh-Kee ;
Sim, Eundeok ;
Kim, Kang-Ho ;
Kim, Jong-Hoi ;
Yun, Ho-Gyeong ;
Kwon, O. Kyun ;
Oh, Kwang Ryong .
ETRI JOURNAL, 2006, 28 (05) :545-554