Melatonin regulates the expression of inflammatory cytokines, VEGF and apoptosis in diabetic retinopathy in rats

被引：27

作者：

Ferreira de Melo, Ismaela Maria ^{[1
]}

Martins Ferreira, Cintia Giselle ^{[1
]}

Lima da Silva Souza, Elton Hugo ^{[2
]}

Almeida, Lecio Leone ^{[3
]}

de Sa, Fabricio Bezerra ^{[1
]}

Cavalcanti Lapa Neto, Clovis Jose ^{[1
]}

Paz de Castro, Matheus Vinicius ^{[1
]}

Teixeira, Valeria Wanderley ^{[1
]}

Coelho Teixeira, Alvaro Aguiar ^{[1
]}

机构：

[1] Univ Fed Rural Pernambuco, Dept Morphol & Anim Physiol, Recife, PE, Brazil

[2] Univ Fed Rural Pernambuco, Dept Vet Med, Recife, PE, Brazil

[3] Cariri Reg Univ, Dept Biol Sci, Crato, Brazil

来源：

CHEMICO-BIOLOGICAL INTERACTIONS | 2020年 / 327卷

关键词：

Apoptosis; Cytokines; Pineal; Rats; Diabetic retinopathy; Angiogenesis; ENDOTHELIAL GROWTH-FACTOR; RETINAL OXIDATIVE STRESS; HIGH-FAT-DIET; NF-KAPPA-B; GLUCOSE-HOMEOSTASIS; LIVER-INJURY; STREPTOZOTOCIN; INSULIN; CELLS; MODULATION;

D O I：

10.1016/j.cbi.2020.109183

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The present study analyzed whether melatonin could mediate the expression of VEGF, IL-6 and TNF-alpha, as well as the apoptotic index in rats with diabetic retinopathy. Fifty Wistar albino rats were divided into the following groups: GC: rats without induction of diabetes by streptozotocin; GD: rats induced to diabetes by streptozotocin and treated with placebo; GDM: rats induced to diabetes by streptozotocin and after confirmation treated with melatonin at a dose of 10 mg/kg for 20 days; GDMS: rats induced to diabetes by streptozotocin and treated simultaneously with melatonin at a dosage of 10 mg/kg for 20 days; GDI: rats induced to diabetes by streptozotocin and after confirmation treated with insulin for 20 days. Diabetes was induced by intraperitoneal injections of streptozotocin (60 mg/kg), and insulin (5 U/day) was administered subcutaneously. For apoptosis TUNEL was used, while for the analysis of VEGF, IL-6 and TNF-alpha. The results showed that the groups that were treated with melatonin decreased the expression of cytokines and VEGF, in addition to apoptosis. Thus, it is concluded that melatonin can regulate the expression of these factors by improving the condition of the retina in diabetic retinopathy.

引用

页数：8

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