Development and Application of Base Editors

被引：55

作者：

Yang, Bei ^{[1
]}

Yang, Li ^{[2
,3
]}

Chen, Jia ^{[3
,4
]}

机构：

[1] ShanghaiTech Univ, Shanghai Inst Adv Immunochem Studies, 393 Middle Huaxia Rd, Shanghai 201210, Peoples R China

[2] ShanghaiTech Univ, Sch Life Sci & Technol, 393 Middle Huaxia Rd, Shanghai 201210, Peoples R China

[3] Chinese Acad Sci, CAS Key Lab Computat Biol, CAS Max Planck Gesell Partner Inst Computat Biol, Shanghai Inst Nutr & Hlth,Shanghai Inst Biol Sci, 320 Yueyang Rd, Shanghai 200031, Peoples R China

[4] Chinese Acad Sci, CAS Ctr Excellence Mol Cell Sci, Shanghai Inst Biochem & Cell Biol, Shanghai, Peoples R China

来源：

CRISPR JOURNAL | 2019年 / 2卷 / 02期

关键词：

CYTOSINE DEAMINATION; DIRECTED EVOLUTION; DNA-POLYMERASES; HUMAN CANCERS; GENOMIC DNA; CRISPR; REPAIR; STRAND; PATHWAY; APOBEC;

D O I：

10.1089/crispr.2019.0001

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Base editing is emerging as a potent new strategy to achieve precise gene editing. By combining different nucleobase deaminases with Cas9 or Cpf1 proteins, several base editors have recently been developed to achieve targeted base conversions in different genomic contexts. Importantly, base editors have been successfully applied in animals, plants, and bacteria to induce precise substitutions at the single-base level with high efficiency. In this review, we summarize recent progress in the development and application of base editors and discuss some of the future directions of the technology.

引用

页码：91 / 104

页数：14

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