Bicelle-enabled structural studies on a membrane-associated cytochrome b5 by solid-state MAS NMR spectroscopy

被引：47

作者：

Xu, Jiadi ^{[1
]}

Duerr, Ulrich H. N. ^{[1
]}

Im, Sang-Choul ^{[2
]}

Gan, Zhehong ^{[3
]}

Waskell, Lucy ^{[2
]}

Ramamoorthy, Ayyalusamy ^{[1
]}

机构：

[1] Univ Michigan, Biophys & Dept Chem, Ann Arbor, MI 48109 USA

[2] Univ Michigan, Dept Anesthesiol, Ann Arbor, MI 48109 USA

[3] Natl High Magnet Field Lab, Tallahassee, FL USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2008年 / 47卷 / 41期

关键词：

bicelles; cytochromes; membrane proteins; NMR spectroscopy; structure elucidation;

D O I：

10.1002/anie.200801338

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Graph Presented) Spinning bicelles: Solving 3D structures of membrane proteins is a great challenge because of the difficulty in finding well-behaved model membranes. Bicelles are well suited to overcome these difficulties and enable the use of solid-state MAS NMR spectroscopy experiments for studies on a large soluble domain containing a low concentration of membrane protein cytochrome b5 at 37°C. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：7864 / 7867

页数：4

共 33 条

[1]

[Anonymous], 2007, ANGEW CHEM, V119, P463

[2] Cation modulation of bicelle size and magnetic alignment as revealed by solid-state NMR and electron microscopy [J].

Arnold, A ;

Labrot, T ;

Oda, R ;

Dufourc, EJ .

BIOPHYSICAL JOURNAL, 2002, 83 (05) :2667-2680

[3] NET POLARIZATION TRANSFER VIA A J-ORDERED STATE FOR SIGNAL ENHANCEMENT OF LOW-SENSITIVITY NUCLEI [J].

BURUM, DP ;

ERNST, RR .

JOURNAL OF MAGNETIC RESONANCE, 1980, 39 (01) :163-168

[4] Magnetically aligned phospholipid bilayers with large q ratios stabilize magnetic alignment with high order in the gel and Lα phases [J].

Cardon, TB ;

Dave, PC ;

Lorigan, GA .

LANGMUIR, 2005, 21 (10) :4291-4298

[5] Towards high-resolution 1H-NMR in biological membranes:: magic angle spinning of bicelles [J].

Carlotti, C ;

Aussenac, F ;

Dufourc, EJ .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2002, 1564 (01) :156-164

[6] J-based 2D homonuclear and heteronuclear correlation in solid-state proteins [J].

Chen, Lingling ;

Kaiser, J. Michael ;

Lai, Jinfeng ;

Polenova, Tatyana ;

Yang, Jun ;

Rienstra, Chad M. ;

Mueller, Leonard J. .

MAGNETIC RESONANCE IN CHEMISTRY, 2007, 45 (S84-S92) :S84-S92

[7] Constant-time through-bond 13C correlation spectroscopy for assigning protein resonances with solid-state NMR spectroscopy [J].

Chen, Lingling ;

Olsen, Ryan A. ;

Elliott, Douglas W. ;

Boettcher, John M. ;

Zhou, Donghua H. ;

Rienstra, Chad M. ;

Mueller, Leonard J. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (31) :9992-9993

[8] Differential line broadening in MAS solid-state NMR due to dynamic interference [J].

Chevelkov, Veniamin ;

Faelber, Katja ;

Schrey, Anna ;

Rehbein, Kristina ;

Diehl, Anne ;

Reif, Bernd .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (33) :10195-10200

[9] The cytochromes P450 and b5 and their reductases -: Promising targets for structural studies by advanced solid-state NMR spectroscopy [J].

Durr, Ulrich H. N. ;

Waskell, Lucy ;

Ramamoorthy, Ayyalusamy .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2007, 1768 (12) :3235-3259

[10] Solid-state NMR reveals structural and dynamical properties of a membrane-anchored electron-carrier protein, cytochrome b5 [J].

Durr, Ulrich H. N. ;

Yamamoto, Kazutoshi ;

Im, Sang-Choul ;

Waskell, Lucy ;

Ramamoorthy, Ayyalusamy .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (21) :6670-+

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