miRNA-218 Inhibits Osteosarcoma Cell Migration and Invasion by Down-regulating of TIAM1, MMP2 and MMP9

被引：109

作者：

Jin, Jie ^{[1
]}

Cai, Lin ^{[1
]}

Liu, Zhi-Ming ^{[2
]}

Zhou, Xue-Song ^{[2
]}

机构：

[1] Wuhan Univ, Zhongnan Hosp, Dept Orthoped, Wuhan 430072, Peoples R China

[2] Tongren Hosp Wuhan, Dept Orthoped, Wuhan, Peoples R China

来源：

ASIAN PACIFIC JOURNAL OF CANCER PREVENTION | 2013年 / 14卷 / 06期

关键词：

miRNA-218; osteosarcoma; TIAM1; proliferation; migration; invasion; MMP; CLINICAL-APPLICATIONS; CANCER; MICRORNAS; PROLIFERATION; GROWTH;

D O I：

10.7314/APJCP.2013.14.6.3681

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Deregulated miRNAs participate in osteosarcoma genesis. In this study, the expression of miRNA-218 in human osteosarcomas, adjacent normal tissues and Saos-2 human osteosarcoma cells was first assessed. Then the precise role of miRNA-218 in osteosarcoma cells was investigated. Upon transfection with a miR-218 expression vector, the proliferation of Saos-2 human osteosarcoma cells determined using the ATPlite assay was significantly suppressed, whilw migration of Saos-2 cells detected by wound healing and invasion determined using transwells were dramatically inhibited. Potential target genes of miR-218 were predicted and T-cell lymphoma invasion and metastasis 1 (TIAM1) and matrix metalloproteinase 2 (MMP2) and 9 (MMP9) were identified. This was confirmed by western blotting, which showed that miR-218 expression inhibited TIAM1, MMP2 and MMP9 protein expression. Collectively, these data suggest that miR-218 acts as a tumor suppressor in osteosarcomas by down-regulating TIAM1, MMP2 and MMP9 expression.

引用

页码：3681 / 3684

页数：4

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