Development of an analytical protocol for the determination of polybrominated biphenyls in water by enzyme-linked immunosorbent assay

被引：0

作者：

Chen, Han-Yu ^{[1
]}

Zhuang, Hui-Sheng ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Sch Environm Sci & Engn, Shanghai 200240, Peoples R China

来源：

RENEWABLE AND SUSTAINABLE ENERGY, PTS 1-7 | 2012年 / 347-353卷

关键词：

Immunoassays; Polybrominated Biphenyls (PBBs); Antigen; Polyclonal Antibody; IMMUNOASSAYS;

D O I：

10.4028/www.scientific.net/AMR.347-353.1537

中图分类号：

TE [石油、天然气工业]; TK [能源与动力工程];

学科分类号：

0807 ; 0820 ;

摘要：

A novel immunoassay has been developed for the quantitative determination of polybrominated biphenyls (PBBs) using indirect competitive format. A new method was developed to synthesize PBBs congener (PCB15) hapten and its artificial immunogen, then the polyclonal antibodies. The assay was optimized concerning the coating conjugate and antibody concentration, incubation time and temperature, the tolerance to organic solvents and so on. Under optimized conditions, PBB15 can be determined in the concentration range of 0.01-100 mu g L-1 with a detection limit of 0.02 mu g L-1. The cross-reactivities of the assays were below 8%. While water samples could be analyzed directly.

引用

页码：1537 / 1541

页数：5