Cloning, tissue expression, and chromosomal assignment of human MRJ gene for a member of the DNAJ protein family

被引：25
作者：
Seki, N
Hattori, A
Hayashi, A
Kozuma, S
Miyajima, N
Saito, T
机构：
[1] Natl Inst Radiol Sci, Genome Res Grp, Inage Ku, Chiba 2638555, Japan
[2] Biotechnol & Med Engn Field, Tokyo, Japan
[3] Kazusa DNA Res Inst, Lab Genome Informat, Chiba, Japan
来源：
JOURNAL OF HUMAN GENETICS | 1999年 / 44卷 / 03期
关键词：
DnaJ; MRJ; MSJ-1; J-domain; chromosome; 11q25;
D O I：
10.1007/s100380050139
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
The DnaJ protein family consists of proteins with a highly conserved amino acid stretch called the "J-domain". A cDNA clone encoding a new protein with a J-domain was isolated from a human fetal brain cDNA library. This new member of the DnaJ family of 241 amino acid residues showed 94% identity with mouse Mrj (accession number, AF035962) and 71% identity with mouse Msj-1 (accession number,U95607) along its entire sequence. Reverse transcription - coupled polymerase chain reaction (RT-PCR) analysis showed the messenger RNA was ubiquitously expressed in various human tissues. The chromosomal location of the gene was determined by PCR-based analyses with both a human/rodent monochromosomal hybrid cell panel and a radiation hybrid panel to map on chromosome 11q25 region.
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收藏
页码：185 / 189
页数：5
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