Serine is a new target residue for endogenous ADP-ribosylation on histones

被引：187

作者：

Leidecker, Orsolya ^{[1
]}

Bonfiglio, Juan Jose ^{[1
]}

Colby, Thomas ^{[1
]}

Zhang, Qi ^{[1
]}

Atanassov, Ilian ^{[1
]}

Zaja, Roko ^{[2
]}

Palazzo, Luca ^{[2
]}

Stockum, Anna ^{[1
]}

Ahel, Ivan ^{[2
]}

Matic, Ivan ^{[1
]}

机构：

[1] Max Planck Inst Biol Ageing, Cologne, Germany

[2] Univ Oxford, Sir William Dunn Sch Pathol, Oxford, England

来源：

NATURE CHEMICAL BIOLOGY | 2016年 / 12卷 / 12期

基金：

英国惠康基金; 欧洲研究理事会;

关键词：

NUDIX HYDROLASES; IDENTIFICATION; PROTEOMICS; CHROMATIN; RIBOSE; SITES; CELLS;

D O I：

10.1038/nchembio.2180

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

ADP-ribosylation (ADPr) is a biologically and clinically important post-translational modification, but little is known about the amino acids it targets on cellular proteins. Here we present a proteomic approach for direct in vivo identification and quantification of ADPr sites on histones. We have identified 12 unique ADPr sites in human osteosarcoma cells and report serine ADPr as a new type of histone mark that responds to DNA damage.

引用

收藏

页码：998 / +

页数：6

相关论文

共 28 条

[1] Immunodot blot method for the detection of poly(ADP-ribose) synthesized in vitro and in vivo [J].

Affar, EB ;

Duriez, PJ ;

Shah, RG ;

Sallmann, FR ;

Bourassa, S ;

Küpper, JH ;

Bürkle, A ;

Poirier, GG .

ANALYTICAL BIOCHEMISTRY, 1998, 259 (02) :280-283

[2] The Promise of Proteomics for the Study of ADP-Ribosylation [J].

Bock, Florian J. ;

Todorova, Tanya T. ;

Chang, Paul .

MOLECULAR CELL, 2015, 58 (06) :911-924

[3] PROTEIN MODIFICATION BY ADP-RIBOSE VIA ACID-LABILE LINKAGES [J].

CERVANTESLAUREAN, D ;

LOFLIN, PT ;

MINTER, DE ;

JACOBSON, EL ;

JACOBSON, MK .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (14) :7929-7936

[4] Andromeda: A Peptide Search Engine Integrated into the MaxQuant Environment [J].

Cox, Juergen ;

Neuhauser, Nadin ;

Michalski, Annette ;

Scheltema, Richard A. ;

Olsen, Jesper V. ;

Mann, Matthias .

JOURNAL OF PROTEOME RESEARCH, 2011, 10 (04) :1794-1805

[5] MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification [J].

Cox, Juergen ;

Mann, Matthias .

NATURE BIOTECHNOLOGY, 2008, 26 (12) :1367-1372

[6] Poly(ADP-ribosyl)ation reactions in the regulation of nuclear functions [J].

D'Amours, D ;

Desnoyers, S ;

D'Silva, I ;

Poirier, GG .

BIOCHEMICAL JOURNAL, 1999, 342 :249-268

[7] Nudix hydrolases degrade protein-conjugated ADP-ribose [J].

Daniels, Casey M. ;

Thirawatananond, Puchong ;

Ong, Shao-En ;

Gabelli, Sandra B. ;

Leung, Anthony K. L. .

SCIENTIFIC REPORTS, 2015, 5

[8] Phosphoproteomic Approach to Characterize Protein Mono- and Poly(ADP-ribosyl)ation Sites from Cells [J].

Daniels, Casey M. ;

Ong, Shao-En ;

Leung, Anthony K. L. .

JOURNAL OF PROTEOME RESEARCH, 2014, 13 (08) :3510-3522

[9] Chromatin to Clinic: The Molecular Rationale for PARP1 Inhibitor Function [J].

Feng, Felix Y. ;

De Bono, Johann S. ;

Rubin, Mark A. ;

Knudsen, Karen E. .

MOLECULAR CELL, 2015, 58 (06) :925-934

[10] A review of tandem mass spectrometry characterization of adenosine diphosphate-ribosylated peptides [J].

Hengel, Shawna M. ;

Goodlett, David R. .

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 2012, 312 :114-121