The interaction between Pseudomonas aeruginosa cells and cationic PC:Chol:DOTAP liposomal vesicles versus outer-membrane structure and envelope properties of bacterial cell

被引：60

作者：

Druis-Kawa, Zuzanna ^{[1
,3
]}

Dorotkiewicz-Jach, Agata ^{[1
]}

Gubernator, Jerzy ^{[2
,4
]}

Gula, Grzegorz ^{[1
]}

Bocer, Tomasz ^{[1
]}

Doroszkiewicz, Wlodzimierz ^{[1
]}

机构：

[1] Univ Wroclaw, Inst Genet & Microbiol, PL-51148 Wroclaw, Poland

[2] Univ Wroclaw, Inst Biochem & Mol Biol, PL-51148 Wroclaw, Poland

[3] Lower Silesian Ctr Paediat Wroclaw, PL-51147 Wroclaw, Poland

[4] Acad Ctr Biotechnol Supramol Lipid Aggregates, PL-51148 Wroclaw, Poland

来源：

INTERNATIONAL JOURNAL OF PHARMACEUTICS | 2009年 / 367卷 / 1-2期

关键词：

Pseudomonas aeruginosa; Outer membrane; Liposomes; Interactions; VITRO ANTIMICROBIAL ACTIVITY; GRAM-POSITIVE BACTERIA; ENHANCED ACTIVITY; LOADED LIPOSOMES; POLYMYXIN-B; FORMULATION; DRUGS; TOBRAMYCIN; MEROPENEM; INFECTION;

D O I：

10.1016/j.ijpharm.2008.09.043

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The interactions between cationic liposomal formulations (PC:Chol:DOTAP 3:43) and 23 Pseudomonas aeruginosa strains were tested. The study was undertaken because different antimicrobial results had been obtained by the authors for Pseudomonas aeruginosa strains and liposomal antibiotics (Drulis-Kawa, Z., Gubernator, J., Dorotkiewicz-Jach, A., Doroszkiewicz, W., Kozubek, A., 2006. The comparison of in vitro antimicrobial activity of liposomes containing meropenem and gentamicin. Cell. Mol. Biol. Lett., 11, 360-375; Drulis-Kawa, Z., Gubernator, J., Dorotkiewicz-Jach, A., Doroszkiewicz W., Kozubek, A., 2006. In vitro antimicrobial activity of liposomal meropenem against Pseudomonas aeruginosa strains. Int. J. Pharm., 315, 59-66). The experiments evaluate the roles of the bacterial outer-membrane structure, especially outer-membrane proteins and LPS, and envelope properties (hydrophobicity and electrostatic potential) in the interactions/fusion process between cells and lipid vesicles. The interactions were examined by fluorescent microscopy using PE-rhodamine-labelled liposomes. Some of the strains exhibited red-light emission (fusion with vesicles or vesicles surrounding the cell) and some showed negative reaction (no red-light emission). The main aim of the study was to determine what kinds of bacterial structure or envelope properties have a major influence on the fusion process. Negatively charged cells and hydrophobic properties promote interaction with cationic lipid vesicles, but no specific correlation was noted for the tested strains. A similar situation concerned LIPS structure, where parent strains and their mutants possessing identical ladder-like band patterns in SDS-PAGE analysis exhibited totally different results with fluorescent microscopy. Outer-membrane protein analysis showed that an 18-kDA protein occurred in the isolates showing fusion with rhodamine-labelled vesicles and, conversely, strains lacking the 18-kDA protein exhibited no positive reaction (red emission). This suggests that even one protein may be responsible for favouring stronger interactions between Pseudomonas aeruginosa cells and cationic liposomal formulations (PC:Chol:DOTAP 3:43). (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：211 / 219

页数：9