Production and characterization of monoclonal antibodies to the steroid receptor coactivator AIB1

被引：10
作者：
Azorsa, DO [1 ]
Meltzer, PS [1 ]
机构：
[1] NHGRI, Canc Genet Branch, NIH, Bethesda, MD 20892 USA
来源：
HYBRIDOMA | 1999年 / 18卷 / 03期
关键词：
D O I：
10.1089/027245799315943
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The steroid coactivator AIB1 (Ampified In Breast cancer 1) is a member of the SRC-1 family of transactivation coactivators that is amplified in about 7% of breast tumor samples. Hybridomas were established that secreted monoclonal antibodies (MAbs) to a recombinant glutathione-S-transferase (GST) fusion protein expressing the steroid receptor interacting domain of AIB1 (GST-AIB.T1: a.a. 605-1294). Four MAbs from these hybridomas were characterized and designated AX15.1, AX15.2, AX15.3, and AX15.4. The MAbs were shown to be specific to AIB1 and did not cross-react with two similar coactivators SRC-1 and TIF2 as shown in Western blot analysis and enzyme-linked immunoadsorbent assay (ELISA), Western blot analysis using the four MAbs showed specific recognition of AIB1 protein as a 160 kDa band in lysates from cell lines containing AIB1 gene-amplification. The MAbs immunoprecipitated in vitro-translated AIB1 and cellular AIB1 from metabolically labeled cells. The results show that these newly described MAbs are useful in studies of AIB1 function and expression.
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收藏
页码：281 / 287
页数：7
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