Synapsable quadruplex-mediated fibers

被引：12

作者：

Angel Mendez, Miguel ^{[1
,2
]}

Szalai, Veronika A. ^{[1
,3
]}

机构：

[1] Univ Maryland Baltimore Cty, Dept Chem & Biochem, Baltimore, MD 21250 USA

[2] Univ San Francisco Quito, Cumbaya 17120084, Quito, Ecuador

[3] NIST, Ctr Nanoscale Sci & Technol, Gaithersburg, MD 20899 USA

来源：

NANOSCALE RESEARCH LETTERS | 2013年 / 8卷

关键词：

Guanine quartet; Guanine quadruplex; Atomic force microscopy; Nanowires; Nanofibers; DNA nanomaterials; Synapsable quadruplex; END-STACKING; FOLDING DNA; G-WIRE; HELICES; REPLICATION; ASSOCIATION; MICROSCOPY; COMPLEX;

D O I：

10.1186/1556-276X-8-210

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

We have fabricated a DNA-based nanofiber created by self-assembly of guanine quadruplex (Hoogsteen base pairing) and double-stranded DNA (Watson-Crick base pairing). When duplexes containing a long stretch of contiguous guanines and single-stranded overhangs are incubated in potassium-containing buffer, the preformed duplexes create high molecular weight species that contain quadruplexes. In addition to observation of these larger species by gel electrophoresis, solutions were analyzed by atomic force microscopy to reveal nanofibers. Analysis of the atomic force microscopy images indicates that fibers form with lengths ranging from 250 to 2,000 nm and heights from 0.45 to 4.0 nm. This work is a first step toward the creation of new structurally heterogeneous (quadruplex/duplex), yet controllable, DNA-based materials exhibiting novel properties suitable for a diverse array of nanotechnology applications.

引用

页码：1 / 10

页数：10

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