LIFETIME ESTIMATION OF MOVING VESICLES IN FREQUENCY-DOMAIN FLUORESCENCE LIFETIME IMAGING MICROSCOPY

被引：0
|
作者：
Roudot, Philippe [1 ]
Kervrann, Charles [1 ]
Waharte, Francois [2 ]
机构：
[1] Inria Rennes Bretagne Atlantique, F-35042 Rennes, France
[2] PICT IBiSA, Inst Curie, CNRS, UMR 144, Paris, France
来源：
2012 9TH IEEE INTERNATIONAL SYMPOSIUM ON BIOMEDICAL IMAGING (ISBI) | 2012年
关键词：
FLIM; frequency-domain; phase modulation; spot tracking; template matching; M-estimation; TRACKING;
D O I：
暂无
中图分类号：
TM [电工技术]; TN [电子技术、通信技术];
学科分类号：
0808 ; 0809 ;
摘要：
We propose a robust statistical framework for correcting the movements of vesicles in frequency domain FLIM imaging. Movement and lifetime are jointly estimated in a three-step procedure. Robust M-estimators are mainly used to improve accuracy in temporally-varying noisy images. The performance of the proposed method is demonstrated on both simulated and real samples.
引用
收藏
页码：668 / 671
页数：4
相关论文
共 50 条
  • [1] LIFETIME MAP RECONSTRUCTION IN FREQUENCY-DOMAIN FLUORESCENCE LIFETIME IMAGING MICROSCOPY
    Roudot, Philippe
    Kervrann, Charles
    Waharte, Francois
    Boulanger, Jerome
    2012 IEEE INTERNATIONAL CONFERENCE ON IMAGE PROCESSING (ICIP 2012), 2012, : 2537 - 2540
  • [2] Lifetime estimation of moving subcellular objects in frequency-domain fluorescence lifetime imaging microscopy
    Roudot, Philippe
    Kervrann, Charles
    Blouin, Cedric M.
    Waharte, Francois
    JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION, 2015, 32 (10) : 1821 - 1835
  • [3] φFLIM:: a new method to avoid aliasing in frequency-domain fluorescence lifetime imaging microscopy
    Van Munster, EB
    Gadella, TWJ
    JOURNAL OF MICROSCOPY-OXFORD, 2004, 213 : 29 - 38
  • [4] Combination of a spinning disc confocal unit with frequency-domain fluorescence lifetime imaging microscopy
    van Munster, E. B.
    Goedhart, J.
    Kremers, G. J.
    Manders, E. M. M.
    Gadella, T. W. J., Jr.
    CYTOMETRY PART A, 2007, 71A (04) : 207 - 214
  • [5] A practical implementation of multifrequency widefield frequency-domain fluorescence lifetime imaging microscopy
    Chen, Hongtao
    Gratton, Enrico
    MICROSCOPY RESEARCH AND TECHNIQUE, 2013, 76 (03) : 282 - 289
  • [6] Linear Behavior of the Phase Lifetime in Frequency-Domain Fluorescence Lifetime Imaging of FRET Constructs
    Sumetsky, Daniel
    Jiang, James Y.
    Ayad, Marina A.
    Mahon, Timothy
    Menaesse, Audrey
    Cararo-Lopes, Marina M.
    Patel, Mihir V.
    Firestein, Bonnie L.
    Boustany, Nada N.
    FRONTIERS IN PHYSICS, 2021, 9 (09):
  • [7] Multiple frequency fluorescence lifetime imaging microscopy
    Squire, A
    Verveer, PJ
    Bastiaens, PIH
    JOURNAL OF MICROSCOPY-OXFORD, 2000, 197 : 136 - 149
  • [8] Endoscopic tissue characterization by frequency-domain fluorescence lifetime imaging (FD-FLIM)
    J. Mizeret
    G. Wagnières
    T. Stepinac
    H. Van Den Bergh
    Lasers in Medical Science, 1997, 12 : 209 - 217
  • [9] Endoscopic tissue characterization by frequency-domain fluorescence lifetime imaging (FD-FLIM)
    Mizeret, J
    Wagnieres, G
    Stepinac, T
    VandenBergh, H
    LASERS IN MEDICAL SCIENCE, 1997, 12 (03) : 209 - 217
  • [10] Super-resolution multiphoton frequency-domain fluorescence lifetime imaging microscopy by generalized stepwise optical saturation (GSOS)
    Zhang, Yide
    Benirschke, David
    Abdalsalam, Ola
    Howard, Scott S.
    SINGLE MOLECULE SPECTROSCOPY AND SUPERRESOLUTION IMAGING XII, 2019, 10884
12345