LIFETIME ESTIMATION OF MOVING VESICLES IN FREQUENCY-DOMAIN FLUORESCENCE LIFETIME IMAGING MICROSCOPY

被引：0
|
作者：
Roudot, Philippe [1 ]
Kervrann, Charles [1 ]
Waharte, Francois [2 ]
机构：
[1] Inria Rennes Bretagne Atlantique, F-35042 Rennes, France
[2] PICT IBiSA, Inst Curie, CNRS, UMR 144, Paris, France
来源：
2012 9TH IEEE INTERNATIONAL SYMPOSIUM ON BIOMEDICAL IMAGING (ISBI) | 2012年
关键词：
FLIM; frequency-domain; phase modulation; spot tracking; template matching; M-estimation; TRACKING;
D O I：
暂无
中图分类号：
TM [电工技术]; TN [电子技术、通信技术];
学科分类号：
0808 ; 0809 ;
摘要：
We propose a robust statistical framework for correcting the movements of vesicles in frequency domain FLIM imaging. Movement and lifetime are jointly estimated in a three-step procedure. Robust M-estimators are mainly used to improve accuracy in temporally-varying noisy images. The performance of the proposed method is demonstrated on both simulated and real samples.
引用
收藏
页码：668 / 671
页数：4
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