1-benzyl-1,4-diazepane reduces the efflux of resistance-nodulation-cell division pumps inEscherichia coli

被引：7

作者：

Casalone, Enrico ^{[1
]}

Vignolini, Tiziano ^{[2
]}

Braconi, Laura ^{[3
]}

Gardini, Lucia ^{[2
,4
]}

Capitanio, Marco ^{[2
,5
]}

Pavone, Francesco S. ^{[2
,4
,5
]}

Dei, Silvia ^{[3
]}

Teodori, Elisabetta ^{[3
]}

机构：

[1] Univ Florence, Dept Biol, Via Madonna del Piano 6, I-50019 Sesto Fiorentino, Italy

[2] LENS European Lab Nonlinear Spect, Via Nello Carrara 1, I-50019 Sesto Fiorentino, Italy

[3] Dept Neurosci Psychol Drug Res & Child Hlth NEURO, Via U Schiff 6, I-650019 Sesto Fiorentino, Italy

[4] CNR, Natl Inst Opt, Largo Fermi 6, I-50125 Florence, Italy

[5] Univ Florence, Dept Phys & Astron, Via Sansone 1, I-50019 Sesto Fiorentino, Italy

来源：

FUTURE MICROBIOLOGY | 2020年 / 15卷 / 11期

基金：

欧盟地平线“2020”;

关键词：

AcrAB expression; E; coli; efflux-pump inhibitor; EPI; levofloxacin potentiation; MDR; membrane permeability; multidrug resistance; ESCHERICHIA-COLI; ANTIBIOTIC-RESISTANCE; MULTIDRUG-RESISTANCE; MAR OPERON; ACRAB; 1-(1-NAPHTHYLMETHYL)-PIPERAZINE; SUSCEPTIBILITY; EXPRESSION; PHENOTYPE; GENES;

D O I：

10.2217/fmb-2019-0296

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Aim:To investigate the action mechanism of 1-benzyl-1,4-diazepane (1-BD) as efflux pump inhibitor (EPI) inEscherichia colimutants: Delta acrABor overexpressing AcrAB and AcrEF efflux pumps.Materials & methods:Effect of 1-BD on: antibiotic potentiation, by microdilution method; membrane functionality, by fluorimetric assays; ethidium bromide accumulation, by fluorometric real-time efflux assay; AcrB expression, by quantitative photoactivated localization microscopy.Results:1-BD decreases the minimal inhibitory concentration of levofloxacin and other antibiotics and increase ethidium bromide accumulation inE. colioverexpressing efflux pumps but not in the Delta acrABstrain. 1-BD increases membranes permeability, without sensibly affecting inner membrane polarity and decreasesacrABtranscription.Conclusion:1-BD acts as an EPI inE. coliwith a mixed mechanism, different from that of major reference EPIs.

引用

页码：987 / 999

页数：13

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