In vitro toxicological characterization of perfluorinated carboxylic acids with different carbon chain lengths

被引:126
作者
Buhrke, Thorsten [1 ]
Kibellus, Anja [1 ]
Lampen, Alfonso [1 ]
机构
[1] Fed Inst Risk Assessment, Dept Food Safety, D-10589 Berlin, Germany
关键词
PFOA; PFCA; HepG2; PPAR; ACTIVATED-RECEPTOR-ALPHA; PERFLUOROALKYL ACIDS; PPAR-ALPHA; NUCLEAR RECEPTORS; DRINKING-WATER; FATTY-ACIDS; MOUSE; RATS; PERFLUOROBUTYRATE; LIVER;
D O I
10.1016/j.toxlet.2013.01.025
中图分类号
R99 [毒物学(毒理学)];
学科分类号
100405 ;
摘要
Perfluorooctanoic acid (PFOA) is in use for the production of fluoropolymers (PFT). Due to its toxic properties it was proposed to replace the substance in its industrial applications by homologous compounds with shorter carbon chain length that were supposed to be less toxic compared to PFOA, however, the smaller PFOA homologs are poorly characterized so far. In this study we have conducted a comparative analysis of the toxicity of perfluorinated carboxylic acids (PFCA) with a carbon chain length ranging from four to twelve carbon atoms. By using the human hepatocarcinoma cell line HepG2 as an in vitro model for human hepatocytes we could show a positive correlation between the carbon chain length of the respective PFCA and its cytotoxicity. There was, however, no indication of an apoptotic mechanism for cytotoxicity. All PFCA under investigation were negative in two independent genotoxicity assays. As PFOA, being a well-known peroxisome proliferator, the other PFCA tested in this study were also shown to activate human peroxisome proliferator-activated receptor alpha (PPAR alpha) with PFOA having the highest potential of PPAR alpha activation. Moreover, the compounds showed weak potential to activate PPAR gamma and hardly activated PPAR delta. Taken together, the in vitro study revealed that PFCA with a shorter carbon chain length seem to be less toxic than PFOA. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:97 / 104
页数:8
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