A C-trachomatis Cloning Vector and the Generation of C-trachomatis Strains Expressing Fluorescent Proteins under the Control of a C-trachomatis Promoter

被引：92

作者：

Agaisse, Herve ^{[1
]}

Derre, Isabelle ^{[1
]}

机构：

[1] Yale Univ, Sch Med, Dept Microbial Pathogenesis, New Haven, CT 06520 USA

来源：

PLOS ONE | 2013年 / 8卷 / 02期

基金：

美国国家卫生研究院;

关键词：

OBLIGATE INTRACELLULAR PATHOGEN; INCLUSION MEMBRANE-PROTEINS; CHLAMYDIA-TRACHOMATIS; COXIELLA-BURNETII; TRANSPOSON MUTAGENESIS; RICKETTSIA-PROWAZEKII; GENE-TRANSFER; IN-VITRO; Q-FEVER; FUSION;

D O I：

10.1371/journal.pone.0057090

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Here we describe a versatile cloning vector for conducting genetic experiments in C. trachomatis. We successfully expressed various fluorescent proteins (i.e. GFP, mCherry and CFP) from C. trachomatis regulatory elements (i.e. the promoter and terminator of the incDEFG operon) and showed that the transformed strains produced wild type amounts of infectious particles and recapitulated major features of the C. trachomatis developmental cycle. C. trachomatis strains expressing fluorescent proteins are valuable tools for studying the C. trachomatis developmental cycle. For instance, we show the feasibility of investigating the dynamics of inclusion fusion and interaction with host proteins and organelles by time-lapse video microscopy.

引用

页数：13

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