A chemosensor to recognize N-acyl homoserine lactone in bacterial biofilm

被引：14

作者：

Das, Sujoy ^{[1
]}

Sarkar, Himadri Sekhar ^{[1
]}

Uddin, Md Raihan ^{[2
]}

Mandal, Sukhendu ^{[2
]}

Sahoo, Prithidipa ^{[1
]}

机构：

[1] Visva Bharati Univ, Dept Chem, Santini Ketan 731235, W Bengal, India

[2] Univ Calcutta, Dept Microbiol, Kolkata 700019, India

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2018年 / 259卷

关键词：

N-acyl homoserine lactone; Chemosensing ensemble; Fluorimetric quantification; DFT calculation; Biofilm imaging; SELECTIVE FLUORESCENT SENSOR; RHODAMINE-BASED FLUORESCENT; AQUEOUS-SOLUTION; MOLECULAR RECOGNITION; SIGNAL MOLECULES; LIVING CELLS; LIVE CELLS; URIC-ACID; QUANTIFICATION; HG2+;

D O I：

10.1016/j.snb.2017.12.040

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple, sensitive, and rapid assay has been developed for detection of N-acyl homoserine lactone (HSL) autoinducers involved in bacterial quorum sensing (QS) by a new coumarin appended rhodamine derivative (RNC) ensembled with Cu2+. RNC-Cu2+ conjugate shows "turn on" fluorescence while binding with HSLs. The interaction of ensemble with HSL is found to be 1:1 stoichiometry on the basis of absorption and fluorescence titrations with the detection limit of 2.4 mu M at neutral pH. NMR, IR titrations and DFT calculations were performed in order to demonstrate the sensing mechanism and the electronic properties of the receptor-donor complex. The present chemosensor improves upon any previous biosensor-based approaches to selectively quantitate HSL, both in cell-free and in whole-cell assay system. (c) 2017 Elsevier B.V. All rights reserved.

引用

页码：332 / 338

页数：7

共 49 条

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