Effective screening for double heterozygosity of Hb E/α0-thalassemia

被引：15

作者：

Sanchaisuriya, Kanokwan ^{[1
]}

Chirakul, Sunisa ^{[1
]}

Srivorakun, Hataichanok ^{[1
]}

Fucharoen, Goonnapa ^{[1
]}

Fucharoen, Supan ^{[1
]}

Changtrakul, Yossombat ^{[2
]}

Sanchaisuriya, Pattara ^{[3
]}

机构：

[1] Khon Kaen Univ, Fac Associated Med Sci, Ctr Res & Dev, Med Diagnost Labs, Khon Kaen 40002, Thailand

[2] Srinagarind Hosp, Clin Microscopy Unit, Fac Med, Khon Kaen, Thailand

[3] Khon Kaen Univ, Fac Publ Hlth, Dept Nutr, Khon Kaen 40002, Thailand

来源：

ANNALS OF HEMATOLOGY | 2008年 / 87卷 / 11期

关键词：

hemoglobin E; alpha-thalassemia; screening strategy; RBC indices;

D O I：

10.1007/s00277-008-0520-x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

One hundred and forty-one blood samples of hemoglobin E (Hb E) carriers were collected to define suitable cutoff values of Hb E level, mean corpuscular volume (MCV), and mean corpuscular hemoglobin (MCH) as screening indicators for detecting individuals with double heterozygosity for Hb E/alpha(0)-thalassemia. Based on the values that gave 100% sensitivity, Hb E<26%, MCV < 74 fl, and MCH < 24 pg were selected. Further validation of these selected values in additional 152 heterozygous Hb E pregnant women revealed 100% sensitivity, 86.2% specificity, and a 25.9% positive predictive value (PPV) for using Hb E cutoff point only, meanwhile, 100% sensitivity, 83.4% specificity, and 22.6% PPV were achieved for the MCV cutoff point. In addition, 100% sensitivity, 86.3% specificity, and 25.9% PPV were gained for the MCH cutoff point. Combining Hb E level with either MCV or MCH cutoff points, the specificity and PPV were increased to 95.1% and 50.0%, respectively. It is concluded that Hb E level < 26%, MCV < 74 fl, and MCH < 24 pg could be used for screening alpha(0)-thalassemia in heterozygous Hb E. However, to improve specificity and PPV of the tests, a combination of Hb E level < 26% with either MCV < 74 fl or MCH < 24 pg is recommended.

引用

页码：911 / 914

页数：4

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