Adsorption and release of rhbFGF from the mesoporous molecular SBA-15 in vitro

被引：0

作者：

Song, Yu ^{[1
]}

Sun, Li Wei ^{[1
]}

Jiang, Rui ^{[1
]}

Feng, Kai ^{[1
]}

Ma, Rui ^{[1
]}

Chen, Xue Nan ^{[1
]}

机构：

[1] Beihua Univ, Dept Med, Jilin 132013, Peoples R China

来源：

ADVANCES IN CHEMICAL ENGINEERING, PTS 1-3 | 2012年 / 396-398卷

关键词：

SBA-15; rhbFGF; Adsorption and release; Cell activity; SILICA;

D O I：

10.4028/www.scientific.net/AMR.396-398.1358

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

A mesoporous molecular SBA-15 was synthesized using Pluronics P123 (PEO20PPO70PEO20) as a structure-directing agent and TEOS as the silica source under acidic condition, and the structure of SBA-15 was determined by X-ray powder diffractometer and N-2 adsorption desorption. As it has been reported that siliceous SBA-15 adsorbents can have a very high affinity and capacity for proteins, the recombinant human basic fibroblast growth factor (rhbFGF) which is a kind of unstable protein was adsorpted by SBA-15, then the properties of SBA-15/bFGF complex were studied. The results had shown that the adsorption rate of rhbFGF by SBA - 15 reached 69.31% in pH 7.4 phosphate buffer at 4 degrees C. After 14 days, the released rate of rhbFGF from SBA-15 was 41.40% and 43.51% respectively in pH 10 phosphate buffer at 4 degrees C and 25 degrees C. Furthermore, the proliferation activity on NIH3T3 cells of the released rhbFGF still can be detected by MTT assay. Therefore, SBA-15 is possible to be used as a kind of material for bFGF adsorption and releasion.

引用

页码：1358 / 1364

页数：7

共 17 条

[1]

Andreas B., 1997, ENDOCRINE SOC, V2, P26

[2] Delivery of basic fibroblast growth factor (bFGF) from photoresponsive hydrogel scaffolds [J].