PDK1 regulates platelet activation and arterial thrombosis

被引:72
作者
Chen, Xue [1 ]
Zhang, Yue [2 ]
Wang, Yanhua [1 ]
Li, Ding [1 ]
Zhang, Lin [1 ]
Wang, Kemin [1 ]
Luo, Xinping [3 ]
Yang, Zhongzhou [4 ]
Wu, Yi [5 ]
Liu, Junling [1 ]
机构
[1] Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, Dept Biochem & Mol Cell Biol,Shanghai Key Lab Tum, Shanghai 200025, Peoples R China
[2] Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, Sino French Res Ctr Life Sci & Genom, Shanghai 200025, Peoples R China
[3] Fudan Univ, Huashan Hosp, Dept Cardiol, Shanghai 200433, Peoples R China
[4] Nanjing Univ, Minist Educ, Key Lab Model Anim Dis Study, Model Anim Res Ctr, Nanjing 210008, Jiangsu, Peoples R China
[5] Soochow Univ, Jiangsu Inst Hematol, Cyrus Tang Hematol Ctr, Affiliated Hosp 1, Suzhou, Peoples R China
基金
中国国家自然科学基金;
关键词
PROTEIN-KINASE-B; GLYCOGEN-SYNTHASE KINASE-3; SIGNALING PATHWAY; TYROSINE KINASE; DISTINCT ROLES; IN-VIVO; PHOSPHORYLATION; AKT; MICE; BINDING;
D O I
10.1182/blood-2012-10-461897
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The effects of phosphoinositide-dependent protein kinase 1 (PDK1), a master kinase in the phosphoinositide 3-kinase/Akt pathway, on platelet activation are unknown. Accordingly, platelet-specific PDK1-deficient mice were characterized to elucidate the platelet-related function(s) of PDK1. We found that PDK1 deficiency caused mild thrombocytopenia. The aggregation of PDK1(-/-) platelets was diminished in response to low levels of thrombin, U46619, and adenosine 5'-diphosphate. Further results demonstrated that PDK1 regulates thrombin-induced platelet activation by affecting alpha IIb beta 3-mediated outside-in signaling. This result provided an explanation for the diminished spreading of PDK1(-/-) platelets on immobilized fibrinogen (Fg) and the decreased rate of clot retraction in platelet-rich plasma (PRP) containing PDK1(-/-) platelets. PDK1 deficiency diminished agonist-induced Akt Ser473 phosphorylation and thoroughly abolished Akt Thr308 and Gsk3 beta Ser9 phosphorylation in response to agonist treatment and platelet spreading, respectively. A Gsk3b inhibitor fully restored the aggregation of PDK1(-/-) platelets in response to low levels of thrombin, normal spreading of PDK1(-/-) platelets on Fg, and normal clot retraction in PRP containing PDK1(-/-) platelets. Those results indicated that Gsk3b is one of the major downstream effectors of PDK1 in thrombin-induced platelet activation and alpha IIb beta 3-mediated outside-in signaling. In addition, in vivo data demonstrated that PDK1 is an important regulator in arterial thrombosis formation.
引用
收藏
页码:3718 / 3726
页数:9
相关论文
共 48 条
[1]   Mechanism of activation of protein kinase B by insulin and IGF-1 [J].
Alessi, DR ;
Andjelkovic, M ;
Caudwell, B ;
Cron, P ;
Morrice, N ;
Cohen, P ;
Hemmings, BA .
EMBO JOURNAL, 1996, 15 (23) :6541-6551
[2]   Role of translocation in the activation and function of protein kinase B [J].
Andjelkovic, M ;
Alessi, DR ;
Meier, R ;
Fernandez, A ;
Lamb, NJC ;
Frech, M ;
Cron, P ;
Cohen, P ;
Lucocq, JM ;
Hemmings, BA .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (50) :31515-31524
[3]   S6K1 and mTOR regulate Rac1-driven platelet activation and aggregation [J].
Aslan, Joseph E. ;
Tormoen, Garth W. ;
Loren, Cassandra P. ;
Pang, Jiaqing ;
McCarty, Owen J. T. .
BLOOD, 2011, 118 (11) :3129-3136
[4]   Regulation of glycogen synthase kinase 3 in human platelets: a possible role in platelet function? [J].
Barry, FA ;
Graham, GJ ;
Fry, MJ ;
Gibbins, JM .
FEBS LETTERS, 2003, 553 (1-2) :173-178
[5]   Protein kinase B is regulated in platelets by the collagen receptor glycoprotein VI [J].
Barry, FA ;
Gibbins, JM .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (15) :12874-12878
[6]   The two TORCs and Akt [J].
Bhaskar, Prashanth T. ;
Hay, Nissim .
DEVELOPMENTAL CELL, 2007, 12 (04) :487-502
[7]   Regulation of FAK Ser-722 phosphorylation and kinase activity by GSK3 and PP1 during cell spreading and migration [J].
Bianchi, M ;
De Lucchini, S ;
Marin, O ;
Turner, DL ;
Hanks, SK ;
Villa-Moruzzi, E .
BIOCHEMICAL JOURNAL, 2005, 391 :359-370
[8]   Phosphoinositide-dependent protein kinase 1, a sensor of protein conformation [J].
Biondi, RM .
TRENDS IN BIOCHEMICAL SCIENCES, 2004, 29 (03) :136-142
[9]   The PIF-binding pocket in PDK1 is essential for activation of S6K and SGK, but not PKB [J].
Biondi, RM ;
Kieloch, A ;
Currie, RA ;
Deak, M ;
Alessi, DR .
EMBO JOURNAL, 2001, 20 (16) :4380-4390
[10]   Platelets Amplify Inflammation in Arthritis via Collagen-Dependent Microparticle Production [J].
Boilard, Eric ;
Nigrovic, Peter A. ;
Larabee, Katherine ;
Watts, Gerald F. M. ;
Coblyn, Jonathan S. ;
Weinblatt, Michael E. ;
Massarotti, Elena M. ;
Remold-O'Donnell, Eileen ;
Farndale, Richard W. ;
Ware, Jerry ;
Lee, David M. .
SCIENCE, 2010, 327 (5965) :580-583