共 48 条
PDK1 regulates platelet activation and arterial thrombosis
被引:72
作者:
Chen, Xue
[1
]
Zhang, Yue
[2
]
Wang, Yanhua
[1
]
Li, Ding
[1
]
Zhang, Lin
[1
]
Wang, Kemin
[1
]
Luo, Xinping
[3
]
Yang, Zhongzhou
[4
]
Wu, Yi
[5
]
Liu, Junling
[1
]
机构:
[1] Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, Dept Biochem & Mol Cell Biol,Shanghai Key Lab Tum, Shanghai 200025, Peoples R China
[2] Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, Sino French Res Ctr Life Sci & Genom, Shanghai 200025, Peoples R China
[3] Fudan Univ, Huashan Hosp, Dept Cardiol, Shanghai 200433, Peoples R China
[4] Nanjing Univ, Minist Educ, Key Lab Model Anim Dis Study, Model Anim Res Ctr, Nanjing 210008, Jiangsu, Peoples R China
[5] Soochow Univ, Jiangsu Inst Hematol, Cyrus Tang Hematol Ctr, Affiliated Hosp 1, Suzhou, Peoples R China
来源:
基金:
中国国家自然科学基金;
关键词:
PROTEIN-KINASE-B;
GLYCOGEN-SYNTHASE KINASE-3;
SIGNALING PATHWAY;
TYROSINE KINASE;
DISTINCT ROLES;
IN-VIVO;
PHOSPHORYLATION;
AKT;
MICE;
BINDING;
D O I:
10.1182/blood-2012-10-461897
中图分类号:
R5 [内科学];
学科分类号:
1002 ;
100201 ;
摘要:
The effects of phosphoinositide-dependent protein kinase 1 (PDK1), a master kinase in the phosphoinositide 3-kinase/Akt pathway, on platelet activation are unknown. Accordingly, platelet-specific PDK1-deficient mice were characterized to elucidate the platelet-related function(s) of PDK1. We found that PDK1 deficiency caused mild thrombocytopenia. The aggregation of PDK1(-/-) platelets was diminished in response to low levels of thrombin, U46619, and adenosine 5'-diphosphate. Further results demonstrated that PDK1 regulates thrombin-induced platelet activation by affecting alpha IIb beta 3-mediated outside-in signaling. This result provided an explanation for the diminished spreading of PDK1(-/-) platelets on immobilized fibrinogen (Fg) and the decreased rate of clot retraction in platelet-rich plasma (PRP) containing PDK1(-/-) platelets. PDK1 deficiency diminished agonist-induced Akt Ser473 phosphorylation and thoroughly abolished Akt Thr308 and Gsk3 beta Ser9 phosphorylation in response to agonist treatment and platelet spreading, respectively. A Gsk3b inhibitor fully restored the aggregation of PDK1(-/-) platelets in response to low levels of thrombin, normal spreading of PDK1(-/-) platelets on Fg, and normal clot retraction in PRP containing PDK1(-/-) platelets. Those results indicated that Gsk3b is one of the major downstream effectors of PDK1 in thrombin-induced platelet activation and alpha IIb beta 3-mediated outside-in signaling. In addition, in vivo data demonstrated that PDK1 is an important regulator in arterial thrombosis formation.
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页码:3718 / 3726
页数:9
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