Expression of PprI from Deinococcus radiodurans Improves Lactic Acid Production and Stress Tolerance in Lactococcus lactis

被引：18
|
作者：
Dong, Xiangrong [1 ]
Tian, Bing [2 ,3 ]
Dai, Shang [2 ,3 ]
Li, Tao [2 ,3 ]
Guo, Linna [1 ]
Tan, Zhongfang [1 ]
Jiao, Zhen [1 ]
Jin, Qingsheng [4 ]
Wang, Yanping [1 ]
Hua, Yuejin [2 ,3 ]
机构：
[1] Zhengzhou Univ, Henan Prov Key Lab Ion Beam Bioengn, Zhengzhou 450052, Peoples R China
[2] Zhejiang Univ, Chinese Minist Agr, Key Lab Nucl Agr Sci, Hangzhou 310029, Zhejiang, Peoples R China
[3] Zhejiang Univ, Inst Nucl Agr Sci, Hangzhou 310029, Zhejiang, Peoples R China
[4] Zhejiang Acad Agr Sci, Inst Crops & Utilizat Nucl Technol, Hangzhou 310021, Zhejiang, Peoples R China
来源：
PLOS ONE | 2015年 / 10卷 / 11期
基金：
中国国家自然科学基金;
关键词：
ESCHERICHIA-COLI; EXTREME RADIORESISTANCE; LACTOBACILLUS-CASEI; RADIATION-DAMAGE; SALINITY STRESS; SALT TOLERANCE; RESISTANCE; REGULATOR; PROTEIN; GENE;
D O I：
10.1371/journal.pone.0142918
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
PprI is a general switch protein that regulates the expression of certain proteins involved in pathways of cellular resistance in the extremophilic bacterium Deinococcus radiodurans. In this study, we transformed pprI into Lactococcus lactis strain MG1363 using the lactococcal shuttle vector pMG36e and investigated its effects on the tolerance and lactic acid production of L. lactis while under stress. PprI was stably expressed in L. lactis as confirmed by western blot assays. L. lactis expressing PprI exhibited significantly improved resistance to oxidative stress and high osmotic pressure. This enhanced cellular tolerance to stressors might be due to the regulation of resistance-related genes (e.g., recA, recO, sodA, and nah) by pprI. Moreover, transformed L. lactis demonstrated increased lactic acid production, attributed to enhanced lactate dehydrogenase activity. These results suggest that pprI can improve the tolerance of L. lactis to environmental stresses, and this transformed bacterial strain is a promising candidate for industrial applications of lactic acid production.
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页数：15
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