Common approaches for efficient method development with immobilised polysaccharide-derived chiral stationary phases

Immobilised polysaccharide-based chiral stationary phases (CSPs) are a new generation of chromatographic materials combining the remarkable enantioselective performance of the polysaccharide derivatives and solvent versatility for enantiomeric resolution. Based on extensive experimental work, this article will focus on the approach to efficient method development with these immobilised CSPs (CHIRALPAK IA, CHIRALPAK IB and CHIRALPAK IC) by applying a limited number of mobile phases. The manuscript will review the development of screening strategies, either by liquid and supercritical chromatography (LC and SFC), for the separation of enantiomers. The rational combination of both modes leads to efficient approaches to get enantiomeric resolutions in reasonable time frames and with high success rates. (c) 2008 Elsevier B.V. All rights reserved.