Preparation, structure and anticoagulant activity of a low molecular weight fraction produced by mild acid hydrolysis of sulfated rhamnan from Monostroma latissimum

被引：44
作者：
Li, Hongyan [1 ]
Mao, Wenjun [1 ]
Hou, Yujiao [1 ]
Gao, Yan [2 ]
Qi, Xiaohui [1 ]
Zhao, Chunqi [1 ]
Chen, Yin [1 ]
Chen, Yanli [1 ]
Li, Na [1 ]
Wang, Chunyan [1 ]
机构：
[1] Ocean Univ China, Inst Marine Drug & Food, Minist Educ, Key Lab Marine Drugs, Qingdao 266003, Peoples R China
[2] Natl Oceanog Ctr Qingdao, Qingdao 266071, Peoples R China
来源：
BIORESOURCE TECHNOLOGY | 2012年 / 114卷
基金：
中国国家自然科学基金;
关键词：
Sulfated rhamnan; Depolymerization; Structure; Anticoagulant activity; Heparin cofactor II; CAULERPA-CUPRESSOIDES; CHEMICAL-STRUCTURE; GREEN-ALGA; POLYSACCHARIDE; GALACTAN; RHODOPHYTA; FUCOIDAN; HEPARIN; FUCANS;
D O I：
10.1016/j.biortech.2012.03.025
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
A low molecular weight fraction, designated LMWP, was prepared by mild acid hydrolysis of sulfated rhamnan from Monostroma latissimum and purified by anion-exchange and gel-permeation chromatography. Chemical and spectroscopic analyses showed that LMWP was mainly composed of rhamnose, and its molecular weight was about 33.6 kDa. The backbone of LMWP consists of 1,3-linked alpha-L-rhamnose units with partially sulfate groups at the C-2 position. Approximately 25% of 1,3-linked alpha-L-rhamnose units is substituted at C-2 by sulfated or non-sulfated 1,3-linked alpha-L-rhamnose and 1,2-linked alpha-L-rhamnose units. LMWP effectively prolonged clotting time as evaluated by the activated partial thromboplastin time assay and was a potent thrombin inhibitor mediated by heparin cofactor II. The investigation demonstrated that LMWP is a novel sulfated polysaccharide with anticoagulant activity. (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码：414 / 418
页数：5
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