Antigenic Liposomes for Generation of Disease-specific Antibodies

被引:15
作者
Bednar, Kyle J. [1 ]
Hardy, Lakeya [2 ,3 ]
Smeekens, Johanna [3 ,4 ]
Raghuwanshi, Dharmendra [5 ]
Duan, Shiteng [6 ]
Kulis, Mike D. [3 ,4 ]
Macauley, Matthew S. [5 ,7 ]
机构
[1] Janssen R&D, Beerse, Belgium
[2] Univ N Carolina, Dept Microbiol & Immunol, Chapel Hill, NC 27515 USA
[3] Univ N Carolina, UNC Food Allergy Initiat, Chapel Hill, NC 27515 USA
[4] Univ N Carolina, Dept Pediat, Chapel Hill, NC 27515 USA
[5] Univ Alberta, Dept Chem, Edmonton, AB, Canada
[6] Scripps Res Inst, Dept Mol Med, San Diego, CA USA
[7] Univ Alberta, Dept Med Microbiol & Immunol, Edmonton, AB, Canada
来源
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | 2018年 / 140期
关键词
Immunology and Infection; Issue; 140; Nanoparticles; Liposomes; Anaphylaxis; Peanut Allergy; Calcium Flux; Flow Cytometry; Antigen Specific; Ara h 2; food allergy; IgE; PEANUT ORAL IMMUNOTHERAPY; EPICUTANEOUS IMMUNOTHERAPY; MYASTHENIA-GRAVIS; B-CELLS; ALLERGY; CD22; NANOPARTICLES; MODELS; IGE;
D O I
10.3791/58285
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Antibody responses provide critical protective immunity to a wide array of pathogens. There remains a high interest in generating robust antibodies for vaccination as well as understand how pathogenic antibody responses develop in allergies and autoimmune disease. Generating robust antigen-specific antibody responses is not always trivial. In mouse models, it often requires multiple rounds of immunizations with adjuvant that leads to a great deal of variability in the levels of induced antibodies. One example is in mouse models of peanut allergies where more robust and reproducible models that minimize mouse numbers and the use of adjuvant would be beneficial. Presented here is a highly reproducible mouse model of peanut allergy anaphylaxis. This new model relies on two key factors: (1) antigen-specific splenocytes are adoptively transferred from a peanut-sensitized mouse into a naive recipient mouse, normalizing the number of antigen-specific memory B-and T-cells across a large number of mice; and (2) recipient mice are subsequently boosted with a strong multivalent immunogen in the form of liposomal nanoparticles displaying the major peanut allergen (Ara h 2). The major advantage of this model is its reproducibility, which ultimately lowers the number of animals used in each study, while minimizing the number of animals receiving multiple injections of adjuvant. The modular assembly of these immunogenic liposomes provides relatively facile adaptability to other allergic or autoimmune models that involve pathogenic antibodies.
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页数:13
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