Prevalence and molecular characterization of Cryptosporidium spp. in dairy calves from 11 farms in Prince Edward Island, Canada

被引：29

作者：

Coklin, Tatjana ^{[1
,2
]}

Uehlinger, Fabienne D. ^{[3
]}

Farber, Jeffrey M. ^{[2
]}

Barkema, Herman W. ^{[3
,4
]}

O'Handley, Ryan M. ^{[3
,5
]}

Dixon, Brent R. ^{[2
]}

机构：

[1] Canadian Food Inspect Agcy, Ottawa, ON K1A 0Y9, Canada

[2] Hlth Canada, Hlth Prod & Food Branch, Food Directorate,Banting Res Ctr, Bur Microbial Hazards,Microbiol Res Div, Ottawa, ON K1A 0K9, Canada

[3] Univ Prince Edward Isl, Atlantic Vet Coll, Dept Hlth Management, Charlottetown, PE C1A 4P3, Canada

[4] Univ Calgary, Fac Vet Med, Dept Prod Anim Hlth, Calgary, AB T2N 4N1, Canada

[5] Murdoch Univ, Sch Vet & Biomed Sci, Environm Biotechnol CRC, Murdoch, WA 6164, Australia

来源：

VETERINARY PARASITOLOGY | 2009年 / 160卷 / 3-4期

关键词：

Cryptosporidium parvum; Dairy calves; Zoonotic transmission; Genotyping; N. SP APICOMPLEXA; GIARDIA-DUODENALIS; PARVUM INFECTION; CATTLE; GENOTYPES; ASSOCIATION; DIARRHEA; ONTARIO; HUMANS; BOVIS;

D O I：

10.1016/j.vetpar.2008.10.096

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Cryptosporidium spp. are common intestinal protozoan parasites that infect a wide range of hosts, including humans and livestock, worldwide. The objective of this study was to determine the prevalence of Cryptosporidium spp. in dairy calves in Prince Edward Island, Canada, and the potential for transmission of this parasite between dairy calves and humans. Fecal samples were collected from 183 dairy calves from I I farms in Prince Edward Island. The prevalence of Cryptosporidium spp. infections in these animals was determined by examining for the presence of oocysts in the fecal samples, using immunofluorescence microscopy. Molecular characterization was done using a nested-PCR protocol to amplify fragments of the Cryptosporidium heat-shock protein 70 gene, followed by DNA sequencing. Ten calves (6.2%), representing 4 out of 11 farms tested, were positive for Cryptosporidium spp. DNA sequence analysis on five PCR positive samples demonstrated that Cryptosporidium parvum was the only species present in the calves tested, suggesting that there is a potential risk of zoonotic transmission between dairy calves and humans in this region. Crown Copyright (C) 2008 Published by Elsevier B.V. All rights reserved.

引用

页码：323 / 326

页数：4

共 33 条

[1] Unravelling Cryptosporidium and Giardia epidemiology [J].

Cacciò, SM ;

Thompson, RCA ;

McLauchlin, J ;

Smith, HV .

TRENDS IN PARASITOLOGY, 2005, 21 (09) :430-437

[2] Prevalence and molecular characterization of Giardia duodenalis and Cryptosporidium spp. in dairy cattle in Ontario, Canada [J].

Coklin, Tatjana ;

Farber, Jeffrey ;

Parrington, Loma ;

Dixon, Brent .

VETERINARY PARASITOLOGY, 2007, 150 (04) :297-305

[3]

DOHOO I, 2003, VET EPIDEMIOLOGIC RE, P531

[4]

Fall A, 2003, J PARASITOL, V89, P399, DOI 10.1645/0022-3395(2003)089[0399:MINART]2.0.CO

[5]

[6] Prevalence of Cryptosporidium, Giardia and Eimeria infections in post-weaned and adult cattle on three Maryland farms [J].

Fayer, R ;

Trout, JM ;

Graczyk, TK ;

Lewis, EJ .

VETERINARY PARASITOLOGY, 2000, 93 (02) :103-112

[7] Cryptosporidium bovis n. sp (Apicomplexa: Cryptosporidiidae) in cattle (Bos taurus) [J].

Fayer, R ;

Santín, M ;

Xiao, LH .

JOURNAL OF PARASITOLOGY, 2005, 91 (03) :624-629

[8] Prevalence of species and genotypes of Cryptosporidium found in 1-2-year-old dairy cattle in the eastern United States [J].

Fayer, R ;

Santín, M ;

Trout, JM ;

Greiner, E .

VETERINARY PARASITOLOGY, 2006, 135 (02) :105-112

[9] Cryptosporidium:: a water-borne zoonotic parasite [J].

Fayer, R .

VETERINARY PARASITOLOGY, 2004, 126 (1-2) :37-56

[10] Cryptosporidium ryanae n. sp (Apicomplexa: Cryptosporidiidae) in cattle (Bos taurus) [J].

Fayer, Ronald ;

Santin, Monica ;

Trout, James M. .

VETERINARY PARASITOLOGY, 2008, 156 (3-4) :191-198

← 1 2 3 4 →