Determination of usnic acid in lichen toxic to elk by liquid chromatography with ultraviolet and tandem mass spectrometry detection

被引：22
作者：
Roach, JAG [1 ]
Musser, SM [1 ]
Morehouse, K [1 ]
Woo, JYJ [1 ]
机构：
[1] US FDA, Ctr Food Safety & Appl Nutr, College Pk, MD 20740 USA
来源：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2006年 / 54卷 / 07期
关键词：
elk deaths; liquid chromatography-tandem mass spectrometry; LC-MS/MS; tumbleweed shield lichen; Usnea barbata; usnic acid; Xanthoparmelia chlorochroa;
D O I：
10.1021/jf052767m
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
Usnic acid is unambiguously confirmed by tandem mass spectrometry (MS/MS) in tumbleweed shield lichen, Xanthoparmelia chlorochroa. The lichen contains 2% usnic acid by liquid chromatography with UV quantification at 282 nm. The UV linear range for usnic acid quantification is from its 4 ng limit of detection to 2 mu g injected. UV signal saturation is recognized by distortion of the usnic acid UV spectrum. Positive ion electrospray-tandem mass spectrometry offers no similar means to recognize quantification data recorded above the linear range of electrospray. Electrospray ionization capacity and matrix effects limit the reliability of tandem mass spectrometry quantification. The combination of UV quantification and MS confirmation provides a reliable analytical method for measuring usnic acid levels in plant material.
引用
收藏
页码：2484 / 2490
页数：7
相关论文
共 31 条
  • [1] Abo-Khatwa A. N., 1996, Natural Toxins, V4, P96, DOI 10.1002/19960402NT7
  • [2] Bartolucci G, 2000, RAPID COMMUN MASS SP, V14, P967, DOI 10.1002/(SICI)1097-0231(20000615)14:11<967::AID-RCM973>3.0.CO
  • [3] 2-C
  • [4] THE SYNTHESIS OF USNIC ACID
    BARTON, DHR
    DEFLORIN, AM
    EDWARDS, OE
    [J]. JOURNAL OF THE CHEMICAL SOCIETY, 1956, (MAR): : 530 - 534
  • [5] BEATH OA, 2006, B AGR EXP STN U WYOM, P12
  • [6] BERTUZZI PF, 1967, J ASSOC OFF ANA CHEM, V50, P623
  • [7] CAVIGLIA AM, 2001, PARMELIA CAPERATA PA, V56, P379
  • [8] A review on usnic acid, an interesting natural compound
    Cocchietto, M
    Skert, N
    Nimis, PL
    Sava, G
    [J]. NATURWISSENSCHAFTEN, 2002, 89 (04) : 137 - 146
  • [9] COLE RB, 1997, ELECTROSPRAY IOIZATI, P133
  • [10] Dayan F. E., 2001, Pesticide Outlook, V12, P229, DOI 10.1039/b110543b
1234