Protein Recognition by an Ensemble of Fluorescent DNA G-Quadruplexes

被引：66

作者：

Margulies, David ^{[1
]}

Hamilton, Andrew D. ^{[1
]}

机构：

[1] Yale Univ, Dept Chem, New Haven, CT 06520 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2009年 / 48卷 / 10期

基金：

美国国家卫生研究院;

关键词：

DNA; fluorescence; proteins; self-assembly; supramolecular chemistry; ARRAY; DISPLACEMENT; RECEPTORS; FRAGMENTS; SENSORS; BINDING; DYES;

D O I：

10.1002/anie.200804887

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Sniffing out proteins: Fluorescent DNA G-quadruplexes have been used for building versatile signaling receptors for proteins in a single solution. Introducing a protein sample to the ensemble results in a unique emission signature for unambiguous identification (see scheme, R=fluorophore). The self-assembled, pattern-based protein detection systems are easily fabricated, have the potential for high-throughput operations, and have the ability to handle small protein samples. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：1771 / 1774

页数：4

共 27 条

[1] [Anonymous], 2005, ANGEW CHEM INT EDIT
[2] Supramolecular analytical chemistry
Anslyn, Eric V.
[J]. JOURNAL OF ORGANIC CHEMISTRY, 2007, 72 (03) : 687 - 699
[3] Ash D, 2007, GENET ENG BIOTECHN N, V27, P38
[4] Pattern-based detection of different proteins using an array of fluorescent protein surface receptors
Baldini, L
Wilson, AJ
Hong, J
Hamilton, AD
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (18) : 5656 - 5657
[5] Dynamic combinatorial libraries of dye complexes as sensors
Buryak, A
Severin, K
[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2005, 44 (48) : 7935 - 7938
[6] Indicator displacement assays as molecular timers
Buryak, Andrey
Zaubitzer, Friederike
Pozdnoukhov, Alexei
Severin, Kay
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2008, 130 (34) : 11260 - +
[7] Pattern-based peptide recognition
Collins, Byron E.
Anslyn, Eric V.
[J]. CHEMISTRY-A EUROPEAN JOURNAL, 2007, 13 (17) : 4700 - 4708
[8] Floriano P.N., 2003, ANGEW CHEM, V115, P2116
[9] Anomalous fluorescence enhancement of Cy3 and Cy3.5 versus anomalous fluorescence loss of Cy5 and Cy7 upon covalent linking to IgG and noncovalent binding to avidin
Gruber, HJ
Hahn, CD
Kada, G
Riener, CK
Harms, GS
Ahrer, W
Dax, TG
Knaus, HG
[J]. BIOCONJUGATE CHEMISTRY, 2000, 11 (05) : 696 - 704
[10] Displacement of protein-bound aptamers with small molecules screened by fluorescence polarization
Hafner, Markus
Vianini, Elena
Albertoni, Barbara
Marchetti, Laura
Gruene, Imke
Gloeckner, Christian
Famulok, Michael
[J]. NATURE PROTOCOLS, 2008, 3 (04) : 579 - 587

← 1 2 3 →