Twelve wheat varieties were studied for their phytic acid, calcium, iron, zinc, copper and phosphorus contents, which respectively ranged between 114-166, 25.1-53.5, 3.41-5.55, 0.71-3.00, 0.65-1.32 and 298-314 mg/100 g. The molar ratios as Ca:Phy, Phy:Fe, Phy:Zn, Phy:Cu were found to be 0.14-0.29, 1.96-3.86, 5.11-20.5 and 13.0-23.9, respectively. Significant dephytinization, ranging between 35.3 and 69.3% in different varieties, was achieved on exogenous enzymatic treatment. Enzymatic dephytinization in the in vitro simulated gastrointestinal digestion resulted in significant increase in the bioavailability of essential minerals. The increase in bioavailability of calcium, iron, zinc and copper on exogenous enzymatic dephytinization ranged between, respectively (x-folds): 1.30-1.96, 1.11-1.52, 1.22-1.78 and 1.11-1.73. (C) 2012 Elsevier Ltd. All rights reserved.