A label-free fluorescent biosensor for the detection of protein kinase activity based on gold nanoclusters/graphene oxide hybrid materials

被引:22
|
作者
Liu, Qing [1 ]
Li, Ning [1 ,2 ]
Wang, Mengke [1 ]
Wang, Lei [1 ]
Su, Xingguang [1 ]
机构
[1] Jilin Univ, Dept Analyt Chem, Coll Chem, Changchun 130012, Jilin, Peoples R China
[2] Jilin Univ, China Japan Union Hosp, Dept Resp, Changchun 130031, Jilin, Peoples R China
基金
中国国家自然科学基金;
关键词
MUA-Au NCs; Graphene oxide; Fluorescence; Protein kinase activity; GRAPHENE QUANTUM DOTS; REAL-TIME ASSAY; SENSITIVE DETECTION; NANOCLUSTERS-CU2+ ENSEMBLE; PHOSPHORYLATION PROTECTION; PHOTOLUMINESCENT PROBES; METAL NANOCLUSTERS; TURN-ON; NANOPARTICLES; INHIBITION;
D O I
10.1016/j.aca.2018.01.053
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Protein kinase (PKA) can regulate many cellular biological processes by phosphorylation substrate peptide or protein. A new fluorescent biosensing method for the detection of PKA activity was developed by using 11-mercaptoundecanoic acid-capped gold nanoclusters (MUA-Au NCs) and graphene oxide (GO) with low background noise. In this strategy, the special designed peptide could be anchored on the surface of MUA-Au NCs by the Au-S bond and also adsorbed on the surface of GO owing to the electrostatic interaction. As a result, the fluorescence of MUA-Au NCs was quenched leading to low background fluorescence due to the forster resonance energy transfer (FRET) between MUA-Au NCs and GO via peptide as a bridge. However, when the substrate peptide was phosphorylated by PKA, the FRET between GO and MUA-Au NCs was disrupted because of the weakened interaction between the phosphorylated peptide and the GO, resulting in recovery of the fluorescence intensity. The developed label-free fluorescence "turn-off-on" method can detect protein kinase activity in the range of 0.6-2.0 U mL(-1) with a detection limit of 0.17 U mL(-1) (3 sigma). The feasibility of this present method for kinase inhibitor screening was also studied by assessment of H-89 kinase inhibition with an IC50 value of 0.049 mu mol L-1. (C) 2018 Published by Elsevier B.V.
引用
收藏
页码:71 / 78
页数:8
相关论文
共 50 条
  • [1] DNA-hosted copper nanoclusters/graphene oxide based fluorescent biosensor for protein kinase activity detection
    Wang, Mengke
    Lin, Zihan
    Liu, Qing
    Jiang, Shan
    Liu, Hua
    Su, Xingguang
    ANALYTICA CHIMICA ACTA, 2018, 1012 : 66 - 73
  • [2] Label-Free DNA Biosensor Based on Reduced Graphene Oxide and Gold Nanoparticles
    Chiticaru, Elena Alina
    Damian, Celina Maria
    Pilan, Luisa
    Ionita, Mariana
    BIOSENSORS-BASEL, 2023, 13 (08):
  • [3] Graphene based electrochemical biosensor for label-free measurement of the activity and inhibition of protein tyrosine kinase
    Li, Bingyu
    Shi, Xinhao
    Gu, Wei
    Zhao, Kai
    Chen, Ningning
    Xian, Yuezhong
    ANALYST, 2013, 138 (23) : 7212 - 7217
  • [4] Peptide-templated gold nanoclusters as a novel label-free biosensor for the detection of protease activity
    Gu, Yi
    Wen, Qian
    Kuang, Yongqing
    Tang, Lijuan
    Jiang, Jianhui
    RSC ADVANCES, 2014, 4 (27): : 13753 - 13756
  • [5] Label-free fluorometric detection of chymotrypsin activity using graphene oxide/nucleic-acid-stabilized silver nanoclusters hybrid materials
    Li, Shuangqin
    Fu, Yuewei
    Ma, Xuejuan
    Zhang, Yaodong
    BIOSENSORS & BIOELECTRONICS, 2017, 88 : 210 - 216
  • [6] Fluorescent aptamer-functionalized graphene oxide biosensor for label-free detection of mercury(II)
    Li, Ming
    Zhou, Xuejiao
    Ding, Weiqiang
    Guo, Shouwu
    Wu, Nianqiang
    BIOSENSORS & BIOELECTRONICS, 2013, 41 : 889 - 893
  • [7] Gold nanocluster-based fluorescent assay for label-free detection of protein kinase and its inhibitors
    Qing Liu
    Weidan Na
    Lei Wang
    Xingguang Su
    Microchimica Acta, 2017, 184 : 3381 - 3387
  • [8] Gold nanocluster-based fluorescent assay for label-free detection of protein kinase and its inhibitors
    Liu, Qing
    Na, Weidan
    Wang, Lei
    Su, Xingguang
    MICROCHIMICA ACTA, 2017, 184 (09) : 3381 - 3387
  • [9] A label-free fluorescent biosensor based on specific aptamer-templated silver nanoclusters for the detection of tetracycline
    Yang, Si
    Li, Chenxi
    Zhan, Hongyan
    Liu, Rong
    Chen, Wenliang
    Wang, Xiaoli
    Xu, Kexin
    JOURNAL OF NANOBIOTECHNOLOGY, 2023, 21 (01)
  • [10] A label-free fluorescent biosensor based on specific aptamer-templated silver nanoclusters for the detection of tetracycline
    Si Yang
    Chenxi Li
    Hongyan Zhan
    Rong Liu
    Wenliang Chen
    Xiaoli Wang
    Kexin Xu
    Journal of Nanobiotechnology, 21