Assessment of genetic diversity of Bacillus spp. isolated from eutrophic fish culture pond

被引：5
作者：
Sen, R. [1 ]
Tripathy, S. [1 ]
Padhi, S. K. [1 ]
Mohanty, S. [1 ]
Maiti, N. K. [1 ]
机构：
[1] Cent Inst Freshwater Aquaculture, Div Fish Hlth Management, Microbiol Unit, Bhubaneswar 751002, Orissa, India
来源：
3 BIOTECH | 2015年 / 5卷 / 04期
关键词：
Molecular marker; HSP60; gene; ITS; PCR-RFLP; Bacillus group; PHOSPHATE-SOLUBILIZING BACTERIA; PHYLOGENETIC ANALYSIS; TRANSCRIBED SPACER; INTERGENIC SPACER; SEQUENCES; IDENTIFICATION; RDNA; COMMUNITY; REGION; PLACE;
D O I：
10.1007/s13205-014-0234-9
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The genus Bacillus comprises of a diverse group with a wide range of nutritional requirements and physiological and metabolic diversity. Their role in nutrient cycle is well documented. 16S rDNA sequences do not always allow the species to be discriminated. In this study 40 Bacillus spp. obtained from fish culture pond and 10 culture type strains were analysed for their genomic diversity by PCR-RFLP of intergenic spacer region of 16S-23S and HSP60 genes. TaqI digestion of PCR products amplified by ITS PCR did not render distinctive RFLP patterns. Numerical analysis of ITS PCR-RFLP pattern differentiated the isolates into 11 clusters. Same species were found to be grouped in different clusters. But PstI digested PCR products amplified from HSP60 gene of the isolates showed distinctive RFLP patterns. The dendrogram constructed from HSP60 PCR-RFLP delineated the isolates into 11 clusters also. All the clusters, except cluster I grouped only one type of species. The results showed that Bacillus spp. could be clearly distinguished by PCR-RFLP of HSP60 gene. Therefore, the HSP60 gene is proposed as an additional molecular marker for discrimination of Bacillus group.
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页码：393 / 400
页数：8
相关论文
共 25 条
[11]   RAPID IDENTIFICATION OF BACTERIA ON THE BASIS OF POLYMERASE CHAIN REACTION-AMPLIFIED RIBOSOMAL DNA SPACER POLYMORPHISMS [J].
JENSEN, MA ;
WEBSTER, JA ;
STRAUS, N .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (04) :945-952
[12]   New approach to phylogenetic analysis of the genus Bifidobacterium based on partial HSP60 gene sequences [J].
Jian, WY ;
Zhu, L ;
Dong, XZ .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2001, 51 :1633-1638
[13]   Phylogenetic study of Staphylococcus and Macrococcus species based on partial hsp60 gene sequences [J].
Kwok, AYC ;
Chow, AW .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2003, 53 :87-92
[14]   Phylogenetic analysis of gastric and enterohepatic Helicobacter species based on partial HSP60 gene sequences [J].
Mikkonen, TP ;
Kärenlampi, RI ;
Hänninen, ML .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2004, 54 :753-758
[15]   MATHEMATICAL-MODEL FOR STUDYING GENETIC-VARIATION IN TERMS OF RESTRICTION ENDONUCLEASES [J].
NEI, M ;
LI, WH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (10) :5269-5273
[16]   Characterization of the 23S and 5S rRNA genes and 23S-5S intergenic spacer region (ITS-2) of Photobacterium damselae [J].
Osorio, CR ;
Collins, MD ;
Romalde, JL ;
Toranzo, AE .
DISEASES OF AQUATIC ORGANISMS, 2004, 61 (1-2) :33-39
[17]   CLONING OF HSP60 (GROEL) OPERON FROM CLOSTRIDIUM-PERFRINGENS USING A POLYMERASE CHAIN-REACTION BASED APPROACH [J].
RUSANGANWA, E ;
SINGH, B ;
GUPTA, RS .
BIOCHIMICA ET BIOPHYSICA ACTA, 1992, 1130 (01) :90-94
[18]   Enhancement of the fertilizer value of rock phosphate engineered through phosphate-solubilizing bacteria [J].
Sahu, SN ;
Jana, BB .
ECOLOGICAL ENGINEERING, 2000, 15 (1-2) :27-39
[19]   Effect of primers hybridizing to Different evolutionarily conserved regions of the small-subunit rRNA gene in PCR-based microbial community analyses and genetic profiling [J].
Schmalenberger, A ;
Schwieger, F ;
Tebbe, CC .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (08) :3557-3563
[20]  
SOKAL ROBERT R., 1958, UNIV KANSAS SCI BULL, V38, P1409
123