Differential involvement of Src family kinases in pervanadate-mediated responses in rat myometrial cells

被引：18

作者：

Boulven, I ^{[1
]}

Robin, P ^{[1
]}

Desmyter, C ^{[1
]}

Harbon, S ^{[1
]}

Leiber, D ^{[1
]}

机构：

[1] Univ Paris 11, CNRS, UMR 8619, Lab Signalisat & Regulat Cellulaires, F-91405 Orsay, France

来源：

CELLULAR SIGNALLING | 2002年 / 14卷 / 04期

关键词：

rat myometrial cells; pervanadate; PDGF; Src kinase; PLC-gamma; 1; ERK;

D O I：

10.1016/S0898-6568(01)00269-8

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We previously described that pervanadate, a potent tyrosine phosphatase inhibitor, induced contraction of rat myometrium via phospholipase (PL) C-gamma1 activation [Biol Reprod 54 (1996) 1383]. In this study, we found that pervanadate induced tyrosine phosphorylation of the platelet-derived growth factor (PDGF)-beta receptor, interaction of the phosphorylated PDGF receptor with the phosphorylated PLC-gamma1, production of inositol phosphates (InsPs), extracellular signal-regulated kinase (ERK) activation and DNA synthesis. All these responses were insensitive to PDGF receptor kinase inhibition or PDGF receptor down-regulation. We showed that Src family kinases were activated by pervanadate, and that InsPs production and phosphorylation of both PLC-gamma1 and the PDGF receptor were blocked by PP1, an Src inhibitor. In contrast, the stimulation of EPK by pervanadate was totally refractory to PP1. These results demonstrated that the activation of Src by pervanadate is involved in PLC-gamma1/InsPs signalling but does not play a major role in ERK activation. (C) 2002 Elsevier Science Inc. All rights reserved.

引用

页码：341 / 349

页数：9

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