Crystallization and preliminary crystallographic study of HIP/PAP, a human C-lectin overexpressed in primary liver cancers

被引：16
作者：
Abergel, C
Chenivesse, S
Stinnakre, MG
Guasco, S
Bréchot, C
Claverie, JM
Devinoy, E
Christa, L
机构：
[1] Inst Biol Struct & Microbiol, CNRS, UMR 1889, F-13402 Marseille 20, France
[2] CHU Necker, INSERM, U370, F-75742 Paris 15, France
[3] CHU Necker, Liver Unit, F-75742 Paris 15, France
[4] INRA, Unite Biol Cellulaire & Mol, F-78352 Jouy En Josas, France
来源：
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 1999年 / 55卷
关键词：
D O I：
10.1107/S0907444999007969
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Human HIP/PAP is an adhesion protein expressed in normal pancreatic and Paneth cells and overexpressed in hepatocellular carcinoma. HIP/PAP was crystallized using the Hampton Research Crystal Screen and SAmBA software to define the optimal crystallization protocol. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), With unit-cell parameters a = 30.73, b = 49.35, c = 92.15 Angstrom and one molecule in the asymmetric unit. Flash-frozen crystals diffract to 1.78 Angstrom resolution using synchrotron. radiation. A molecular-replacement solution was obtained using the human Reg/ lithostathine structure and the AMoRe software.
引用
收藏
页码：1487 / 1489
页数：3
相关论文
共 21 条
  • [1] Audic S, 1997, PROTEINS, V29, P252, DOI 10.1002/(SICI)1097-0134(199710)29:2<252::AID-PROT12>3.0.CO
  • [2] 2-N
  • [3] THE CCP4 SUITE - PROGRAMS FOR PROTEIN CRYSTALLOGRAPHY
    BAILEY, S
    [J]. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1994, 50 : 760 - 763
  • [4] Crystal structure of human lithostathine, the pancreatic inhibitor of stone formation
    Bertrand, JA
    Pignol, D
    Bernard, JP
    Verdier, JM
    Dagorn, JC
    FontecillaCamps, JC
    [J]. EMBO JOURNAL, 1996, 15 (11) : 2678 - 2684
  • [5] XDL-VIEW, AN X-WINDOWS-BASED TOOLKIT FOR CRYSTALLOGRAPHIC AND OTHER APPLICATIONS
    CAMPBELL, JW
    [J]. JOURNAL OF APPLIED CRYSTALLOGRAPHY, 1995, 28 : 236 - 242
  • [6] HIP/PAP is an adhesive protein expressed in hepatocarcinoma, normal Paneth, and pancreatic cells
    Christa, L
    Carnot, F
    Simon, MT
    Levavasseur, F
    Stinnakre, MG
    Lasserre, C
    Thepot, D
    Clement, B
    Devinoy, E
    Brechot, C
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 1996, 271 (06): : G993 - G1002
  • [7] THE HUMAN HIP GENE, OVEREXPRESSED IN PRIMARY LIVER-CANCER ENCODES FOR A C-TYPE CARBOHYDRATE-BINDING PROTEIN WITH LACTOSE BINDING-ACTIVITY
    CHRISTA, L
    FELIN, M
    MORALI, O
    SIMON, MT
    LASSERRE, C
    BRECHOT, C
    SEVE, AP
    [J]. FEBS LETTERS, 1994, 337 (01) : 114 - 118
  • [8] CA2+-DEPENDENT CARBOHYDRATE-RECOGNITION DOMAINS IN ANIMAL PROTEINS
    DRICKAMER, K
    [J]. CURRENT OPINION IN STRUCTURAL BIOLOGY, 1993, 3 (03) : 393 - 400
  • [9] THE PANCREATITIS ASSOCIATED PROTEIN-III (PAP-III), A NEW MEMBER OF THE PAP GENE FAMILY
    FRIGERIO, JM
    DUSETTI, NJ
    GARRIDO, P
    DAGORN, JC
    IOVANNA, JL
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1993, 1216 (02) : 329 - 331
  • [10] CLONING AND TISSUE-SPECIFIC EXPRESSION OF CDNAS FOR THE HUMAN AND MOUSE HOMOLOGS OF RAT PANCREATITIS-ASSOCIATED PROTEIN (PAP)
    ITOH, T
    TERAOKA, H
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1993, 1172 (1-2) : 184 - 186
123