How low can you go? Pushing the limits of low-input ChIP-seq

被引：8

作者：

Dahl, John Arne ^{[1
]}

Gilfillan, Gregor D. ^{[1
]}

机构：

[1] Oslo Univ Hosp, Oslo, Norway

来源：

BRIEFINGS IN FUNCTIONAL GENOMICS | 2018年 / 17卷 / 02期

关键词：

chromatin immunoprecipitation; HTS; NGS; epigenetics; sequencing; PROTEIN-DNA INTERACTIONS; GENOME-WIDE MAPS; CHROMATIN IMMUNOPRECIPITATION; TRANSCRIPTION FACTORS; MICROFLUIDIC DEVICE; DISTINCT FEATURES; HUMAN-CELLS; IN-VIVO; H3K4ME3; MOUSE;

D O I：

10.1093/bfgp/elx037

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In the past decade, chromatin immunoprecipitation sequencing (ChIP-seq) has emerged as the dominant technique for those wishing to perform genome-wide protein: DNA profiling. Owing to the tissue-and cell-type-specific nature of epigenetic marks, the field has been driven towards obtaining data from ever-lower cell numbers. In this review, we focus on the methodological developments that have lowered input requirements and the biological findings they have enabled, as we strive towards the ultimate goal of robust single-cell ChIP-seq.

引用

页码：89 / 95

页数：7

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