A water-soluble and highly specific fluorescent probe with large Stokes shift for imaging basal HOCl in living cells and zebrafish

被引：29

作者：

Liu, Caiyun ^{[1
]}

Jia, Pan ^{[1
]}

Zhuang, Zihan ^{[1
]}

Wang, Zuokai ^{[1
]}

Duan, Qingxia ^{[1
]}

Li, Zilu ^{[1
]}

Zhu, Hanchuang ^{[1
]}

Zhang, Xue ^{[1
]}

Zhu, Baocun ^{[1
]}

Sheng, Wenlong ^{[2
]}

机构：

[1] Univ Jinan, Shandong Prov Engn Technol Res Ctr Ecol Carbon Si, Sch Water Conservancy & Environm, Jinan 250022, Shandong, Peoples R China

[2] Shandong Acad Sci, Biol Inst, Qilu Univ Technol, 19 Keyuan Rd, Jinan 250014, Shandong, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2019年 / 291卷

基金：

中国国家自然科学基金;

关键词：

Fluorescent probe; Dimethylthiocarbamate (DMTC) moiety; Hypochlorous acid (HOCl); Bioimaging; HYPOCHLOROUS ACID; REACTIVE OXYGEN; NATIVE HOCL; LIVE CELLS; MITOCHONDRIA; NITROGEN; STRESS; SENSOR; SIGNAL;

D O I：

10.1016/j.snb.2019.04.068

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Hypochlorous acid (HOCl) as a biologically important reactive oxygen species (ROS) plays crucial roles in the physiological and pathological processes. It is thus meaningful to explore an effective method for detecting basal HOCl in living systems. We herein report a large Stokes shift (about 115 nm) fluorescent probe LSS-HA for highly specific and ultrasensitive detection of HOCl with excellent water-solubility. Probe LSS-HA exhibited a remarkable fluorescence enhancement ((similar to) 227-fold) due to the adoption of high-quantum-yield fluorophore in aqueous solution. Besides, probe LSS-HA showed ultrasensitivity (detection limit is 3.30 nM) and fast response ( < 5 s) for HOCl. Furthermore, probe LSS-HA was successfully applied for imaging basal HOCl in living cells and zebrafish.

引用

页码：243 / 249

页数：7

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