The analytical application and spectral investigation of DNA-CPB-emodin and sensitive determination of DNA by resonance Rayleigh light scattering technique

被引：10

作者：

Bi, Shuyun ^{[1
]}

Wang, Yu ^{[1
]}

Wang, Tianjiao ^{[1
]}

Pang, Bo ^{[1
]}

Zhao, Tingting ^{[1
]}

机构：

[1] Changchun Normal Univ, Coll Chem, Changchun 130032, Peoples R China

来源：

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2013年 / 101卷

关键词：

Resonance Rayleigh light scattering (RLS); Emodin; CPB; DNA; NUCLEIC-ACIDS; CETYLPYRIDINE BROMIDE; NANOGRAM LEVELS; ASSAY; ENHANCEMENT; PORPHYRIN; SYSTEM; NANOPARTICLES; PROBE; BLUE;

D O I：

10.1016/j.saa.2012.09.088

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

A new sensitive DNA probe containing cetylpyridinium bromide (CPB) and emodin (an effective component of Chinese herbal medicine) was developed using the resonance Rayleigh light scattering (RLS) technique. A novel assay was first developed to detect DNA at nanogram level based on the ternary system of DNA-CPB-emodin. The RLS signal of DNA was enhanced remarkably in the presence of emodin-CPB, and the enhanced RLS intensity at 340.0 nm was in direct proportion to DNA concentration in the range of 0.01-2.72 mu g mL(-1) with a good linear relationship. The detection limit was 1.5 ng mL(-1). Three synthetic DNA samples were measured obtaining satisfactory results, the recovery was 97.6-107.3%. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：233 / 238

页数：6

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