In vitro characterization of a (E)-β-farnesene synthase from Matricaria recutita L. and its up-regulation by methyl jasmonate

被引:26
作者
Su, Shanshan [1 ]
Liu, Xueyan [1 ]
Pan, Guifang [1 ]
Hou, Xiaojuan [1 ]
Zhang, Huimin [1 ]
Yuan, Yi [1 ]
机构
[1] Anhui Agr Univ, Coll Life Sci, Hefei 230036, Peoples R China
关键词
(E)-beta-farnesene synthase; Sesquiterpene; Methyl jasmonate; Matricaria recutita L; MENTHA X PIPERITA; SESQUITERPENE SYNTHASE; BACTERIAL EXPRESSION; MOLECULAR-CLONING; CHAMOMILE; (-)-ALPHA-BISABOLOL; BIOSYNTHESIS; GENE;
D O I
10.1016/j.gene.2015.06.037
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
(E)-beta-farnesene is a sesquiterpene semiochemical that is used extensively by both plants and animals for communication. This acyclic olefin is found in the essential oil of chamomile (Matricaria recutita) and was demonstrated that it could attract natural enemies to reduce cabbage aphids in the Chinese cabbage fields. However, little is known regarding the sequence and function of (E)-beta-farnesene synthase in M. recutita. In this study, we reported a new full-length cDNA encoding (E)-beta-farnesene synthase from M. recutita (Mr-beta FS). The cDNA of Mr-beta FS consisted of 2010 bp including 1725 bp of coding sequence encoding a protein of 574 amino acids with a molecular weight of 67 kDa. The deduced amino acid sequence exhibits a considerably higher homology to beta FS from Artemisia annua (about 92% identity) than to beta FSs from other plants (about 20-40% identity). The recombinant enzyme, produced in Escherichia coli, catalyzed the formation of a single product, (E)-beta-farnesene, from farnesyl diphosphate. Real-time quantitative PCR (qRT-PCR) analysis showed that Mr-beta FS expression was highest in leaves and lowest in disk florets. The treatment of M. recutita with methyl jasmonate (MeJA) significantly enhanced the transcriptional level of beta FS gene and the content of (E)-beta-farnesene in M. recutita. The transcriptional level of beta FS gene was approximately 11.5-fold higher than the control sample and the (E)-beta-farnesene emission level ranged from approximately from 0.082 to 0.695 mu g/g after 24 h induction. Our results laid a solid foundation for later improving crop aphid resistance by transgenic technology and provided an important basic data for the regulation of valuable products from M. recutita. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码:58 / 64
页数:7
相关论文
共 33 条
[1]   Induction of pilocarpine formation in jaborandi leaves by salicylic acid and methyljasmonate [J].
Avancini, G ;
Abreu, IN ;
Saldaña, MDA ;
Mohamed, RS ;
Mazzafera, P .
PHYTOCHEMISTRY, 2003, 63 (02) :171-175
[2]   Response of economically important aphids to components of Hemizygia petiolata essential oil [J].
Bruce, TJA ;
Birkett, MA ;
Blande, J ;
Hooper, AM ;
Martin, JL ;
Khambay, B ;
Prosser, I ;
Smart, LE ;
Wadhams, LJ .
PEST MANAGEMENT SCIENCE, 2005, 61 (11) :1115-1121
[3]   Germacrene C synthase from Lycopersicon esculentum cv. VFNT Cherry tomato:: cDNA isolation, characterization, and bacterial expression of the multiple product sesquiterpene cyclase [J].
Colby, SM ;
Crock, J ;
Dowdle-Rizzo, B ;
Lemaux, PG ;
Croteau, R .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (05) :2216-2221
[4]   Isolation and bacterial expression of a sesquiterpene synthase cDNA clone from peppermint (Mentha x piperita, L.) that produces the aphid alarm pheromone (E)-beta-farnesene [J].
Crock, J ;
Wildung, M ;
Croteau, R .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (24) :12833-12838
[5]   The functional significance of E-β-Farnesene: Does it influence the populations of aphid natural enemies in the fields? [J].
Cui, Liang-Liang ;
Francis, Frederic ;
Heuskin, Stephanie ;
Lognay, Georges ;
Liu, Ying-Jie ;
Dong, Jie ;
Chen, Ju-Lian ;
Song, Xu-Ming ;
Liu, Yong .
BIOLOGICAL CONTROL, 2012, 60 (02) :108-112
[6]   APHID SEMIOCHEMICALS - A REVIEW, AND RECENT ADVANCES ON THE SEX-PHEROMONE [J].
DAWSON, GW ;
GRIFFITHS, DC ;
MERRITT, LA ;
MUDD, A ;
PICKETT, JA ;
WADHAMS, LJ ;
WOODCOCK, CM .
JOURNAL OF CHEMICAL ECOLOGY, 1990, 16 (11) :3019-3030
[7]   Monoterpene and sesquiterpene synthases and the origin of terpene skeletal diversity in plants [J].
Degenhardt, Joerg ;
Koellner, Tobias G. ;
Gershenzon, Jonathan .
PHYTOCHEMISTRY, 2009, 70 (15-16) :1621-1637
[8]  
GASIC O, 1983, Fitoterapia, V54, P51
[9]   CARDIAC EFFECTS OF CHAMOMILE TEA [J].
GOULD, L ;
REDDY, CVR ;
GOMPRECHT, RF .
JOURNAL OF CLINICAL PHARMACOLOGY, 1973, 13 (11-1) :475-479
[10]   Optimisation of a semiochemical slow-release alginate formulation attractive towards Aphidius ervi Haliday parasitoids [J].
Heuskin, Stephanie ;
Lorge, Stephanie ;
Godin, Bruno ;
Leroy, Pascal ;
Frere, Isabelle ;
Verheggen, Francois J. ;
Haubruge, Eric ;
Wathelet, Jean-Paul ;
Mestdagh, Michele ;
Hance, Thierry ;
Lognay, Georges .
PEST MANAGEMENT SCIENCE, 2012, 68 (01) :127-136