Standardization of CalyculinA induced PCC assay and its advantages over Okadaic acid PCC assay in Biodosimetry applications

Objective: In the present study an attempt was made to estimate coefficients of dose response curves for PCC aberrations induced by CalyculinA and Okadaic acid, using Co-60 gamma radiation and 8 MeV pulsed electron beam for biodosimetry application. Materials and Methods: The modified method outlined by Puig et al. 2013 was used to conduct Calyculin A and Okadaic acid induced PCC assay in human blood lymphocytes. Chemical treatment was given for the last 1 h of a 48 h culture. The study was carried out in the dose range 2.5 to 20 Gy using Co-60 gamma rays and 8 MeV pulsed electron beam. Results and Conclusions: Results show a linear dose dependent increase with a slope of 0.047 +/- 0.001 from Calycalin A PCC and 0.048 +/- 0.002 form Okadaic acid PCC. The slope of the fragments curve was 0.327 +/- 0.006 from Calyculin A and 0.328 +/- 0.006 from Okadaic acid PCC. Further, dose calibration studies were carried out for 8 MeV electron using Calyculin A PCC assay and the obtained slope from ring yield was 0.054 +/- 0.002 and 0.427 +/- 0.009 from fragment yield.