Detection of Escherichia coli O157:H7 in ground beef using immunomagnetic separation and multiplex PCR

被引：42

作者：

Sarimehmetoglu, Belgin ^{[1
]}

Aksoy, Mihriban Hatun ^{[2
]}

Ayaz, Naim Deniz ^{[1
]}

Ayaz, Yidiz ^{[2
]}

Kuplulu, Ozlem ^{[1
]}

Kaplan, Yusuf Ziya ^{[2
]}

机构：

[1] Ankara Univ, Fac Vet Med, Dept Food Hyg & Technol, TR-06110 Ankara, Turkey

[2] Etlik Cent Vet Control & Res Inst, TR-06020 Ankara, Turkey

来源：

FOOD CONTROL | 2009年 / 20卷 / 04期

关键词：

E. coli O157:H7; Ground beef; Multiplex PCR; RAW BEEF; VIRULENCE FACTORS; CATTLE FECES; PREVALENCE; O157-H7; MEATS; O157/H7; STRAINS; PRODUCTS; VTEC;

D O I：

10.1016/j.foodcont.2008.06.002

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

In the study 251 fresh ground beef samples sold in Ankara were analyzed to evaluate the prevalence of Escherichia coli O157:H7 by immunomagnetic separation (IMS) based cultivation technique. Virulence factors of the isolates were determined by multiplex PCR. Nineteen (7.6%) of 251 ground beef samples were found as contaminated with E. coli O157. By PCR analyse in two of the isolates fliC(h7) gene was detected and identified as E. coli O157:H7. According to the multiplex PCR one of the isolate has all stx(1), stx(2), eaeA, hly and fliC(h7) genes and the other has stx(1), eaeA, hly and fliCh7 genes. (c) 2008 Elsevier Ltd. All rights reserved.

引用

页码：357 / 361

页数：5

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