Inhibitory effect of verbascoside on xanthine oxidase activity

被引：48

作者：

Wan, Yin ^{[1
,2
]}

Zou, Bin ^{[1
]}

Zeng, Hailong ^{[1
]}

Zhang, Lunning ^{[3
]}

Chen, Ming ^{[4
]}

Fu, Guiming ^{[1
,2
]}

机构：

[1] Nanchang Univ, State Key Lab Food Sci & Technol, Nanchang 330047, Peoples R China

[2] Nanchang Univ, Sch Food Sci & Technol, Nanchang 330047, Peoples R China

[3] Inst Food & Drug Control, Pingxiang 337055, Peoples R China

[4] Nanchang Univ, Affiliated Hosp 1, Nanchang 330006, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2016年 / 93卷

基金：

中国国家自然科学基金;

关键词：

Verbascoside; Xanthine oxidase; Inhibitory activity; Fluorescence chromatographic; Circular dichroism spectra; Molecular simulation; BOVINE SERUM-ALBUMIN; ACTEOSIDE; FLAVONOIDS; MECHANISM;

D O I：

10.1016/j.ijbiomac.2016.09.022

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, we analyzed the inhibitory effect of verbascoside against xanthine oxidase (XOD) in vitro by using animal model and in vivo by direct inhibition assay. Results showed that verbascoside could reduce uric acid in rat serum and inhibit XOD activity in rat liver. The IC50 value of restraining XOD activity was 81.11 mg mL(-1). Fluorescence chromatographic analysis and circular dichroism spectroscopy indicated that the secondary structures of XOD were changed after incubation with verbascoside. The docking simulation showed that verbascoside could enter into the active site of XOD and form hydrogen bonding with amino acid residues (such as Lys-1045, Arg-880, Arg-912, Glu-1261 and Gln-1194). The results suggested that verbascoside, which is a naturally occurring water-soluble antioxidant, could be a potential low-toxicity XOD inhibitor for hyperuricemia treatment. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：609 / 614

页数：6

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