Application of a Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Cow Components Adulterated in Buffalo Milk/Meat

被引：38

作者：

Deb, Rajib ^{[1
]}

Sengar, Gyanendra Singh ^{[1
]}

Singh, Umesh ^{[1
]}

Kumar, Sushil ^{[1
]}

Alyethodi, R. R. ^{[1
]}

Alex, Rani ^{[1
]}

Raja, T. V. ^{[1
]}

Das, A. K. ^{[1
]}

Prakash, B. ^{[1
]}

机构：

[1] ICAR Cent Inst Res Cattle, Mol Genet Lab, Grass Farm Rd, Meerut 250001, Uttar Pradesh, India

来源：

MOLECULAR BIOTECHNOLOGY | 2016年 / 58卷 / 12期

关键词：

LAMP assay; Cow components; Adulteration; Buffalo milk/meat samples; POLYMERASE-CHAIN-REACTION; GOATS MILK; REAL-TIME; IDENTIFICATION; QUANTIFICATION; CHEESE; GENE; DNA;

D O I：

10.1007/s12033-016-9984-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Loop-mediated isothermal amplification (LAMP) is a diagnostic method for amplification of DNA with rapid and minimal equipment requirement. In the present study, we applied the LAMP assay for rapid detection of cow components adulteration in buffalo milk/meat samples. The test can be completed within around 1 h 40 min starting from DNA extraction and can be performed in water bath without requirement of thermocycler. The cow DNA in buffalo samples were identified in the developed LAMP assay by either visualizing with SYBR Green I/HNB dyes or observing the typical ladder pattern on gel electrophoresis. The test can detect up to 5 % level of cow milk/meat mixed in buffalo counterparts. Due to the simplicity and specificity, the developed LAMP test can be easily adapted in any laboratory for rapid detection of cow species identification in livestock by products.

引用

页码：850 / 860

页数：11

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